Ultrasound-assisted extraction of soy isoflavones

被引：454

作者：

Rostagno, MA ^{[1
]}

Palma, M ^{[1
]}

Barroso, CG ^{[1
]}

机构：

[1] Univ Cadiz, Fac Ciencias, Dept Quim Analit, Cadiz 11510, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1012卷 / 02期

关键词：

ultrasound-assisted extraction; extraction methods; soybean; isoflavones;

D O I：

10.1016/S0021-9673(03)01184-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Efficiency in extracting four isoflavone derivatives (daidzin, glycitin, genistin and malonyl genistin) from freeze-dried ground soybeans was compared for mix-stirring extraction and ultrasound-assisted extraction, using different solvents and extraction temperatures with both. The efficiency of the extraction of soy isoflavones was improved by ultrasound but was dependent on the solvent employed. Optimization of the ratios of sample quantity to solvent volume and length of extraction time was also performed. Isoflavones can be quantitatively extracted from soybeans with 50% ethanol at 60 degreesC using ultrasound-assisted extraction in 20 min. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：119 / 128

页数：10

共 18 条

[1] Reactions of genistein with alkylperoxyl radicals [J].

Arora, A ;

Valcic, S ;

Cornejo, S ;

Nair, MG ;

Timmermann, BN ;

Liebler, DC .

CHEMICAL RESEARCH IN TOXICOLOGY, 2000, 13 (07) :638-645

[2] Fast determination of flavonoids in Glycyrrhizae radix by capillary zone electrophoresis [J].

Bo, T ;

Li, KA ;

Liu, HW .

ANALYTICA CHIMICA ACTA, 2002, 458 (02) :345-354

[3] ALAMIN: a chemometric program to check analytical method performance and to assess the trueness by standard addition methodology [J].

Campana, AMG ;

Rodriguez, LC ;

Barrero, FA ;

Ceba, MR .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1997, 16 (07) :381-385

[4]

CHANDRA A, 1996, PHYTOCHEM ANALYSIS, V4, P259

[5] Improved methods for the extraction and analysis of isoflavones from soy-containing foods and nutritional supplements by reversed-phase high-performance liquid chromatography and liquid chromatography-mass spectrometry [J].

Griffith, AP ;

Collison, MW .

JOURNAL OF CHROMATOGRAPHY A, 2001, 913 (1-2) :397-413

[6] Changes in the profile of genistein, daidzein, and their conjugates during thermal processing of tofu [J].

Grün, IU ;

Adhikari, K ;

Li, CQ ;

Li, Y ;

Lin, B ;

Zhang, JL ;

Fernando, LN .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (06) :2839-2843

[7] Development and validation of an isocratic high-performance liquid chromatographic method for quantitative determination of phytoestrogens in soya bean [J].

Hutabarat, LS ;

Mulholland, M ;

Greenfield, H .

JOURNAL OF CHROMATOGRAPHY A, 1998, 795 (02) :377-382

[8] Daidzein and genistein content of fruits and nuts [J].

Liggins, J ;

Bluck, LJC ;

Runswick, S ;

Atkinson, C ;

Coward, WA ;

Bingham, SA .

JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2000, 11 (06) :326-331

[9] Extraction and quantification of daidzein and genistein in food [J].

Liggins, J ;

Bluck, LJC ;

Coward, WA ;

Bingham, SA .

ANALYTICAL BIOCHEMISTRY, 1998, 264 (01) :1-7

[10]

Messina MJ, 1999, AM J CLIN NUTR, V70, p439S, DOI 10.1093/ajcn/70.3.439s

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