Structure of a polysaccharide from Gastrodia elata Bl., and oligosaccharides prepared thereof with anti-pancreatic cancer cell growth activities

被引：117

作者：

Chen, Xia ^{[1
]}

Cao, Dianxiu ^{[1
]}

Zhou, Ling ^{[1
]}

Jin, Hongying ^{[2
]}

Dong, Qun ^{[1
]}

Yao, Jian ^{[1
]}

Ding, Kan ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2011年 / 86卷 / 03期

关键词：

Gastrodia elata Bl; Poly-/oligosaccharide; Anti-cancer; Pancreatic cancer; ANTITUMOR-ACTIVITY; SODIUM-HYDROXIDE; BODIES;

D O I：

10.1016/j.carbpol.2011.06.029

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

To discover anti-pancreatic cancer cells growth inhibitor, a new water-soluble glucan, WTMA, was isolated from the rhizome of Gastrodia elate Bl. The mean molecular weight of the polysaccharide was estimated to be 7.0 x 10(5) Da by high performance gel permeation chromatography (HPGPC). The structure of WTMA was characterized by GC, GC-MS, NMR and MALDI-TOF. WTMA was deduced as an alpha-(1 --> 4)-glucan with alpha-(1 --> 4) linked branches attached to O-6 at branch points. Bioactivity tests in vitro indicated that WTMA and its partial acid hydrolysis-derived mixture product, WTMA-AD-O, could significantly inhibit the proliferation of pancreatic cancer cells. However, WTMA-DE, the degraded polysaccharide without branch chain by isoamylase, did not inhibit the proliferation of pancreatic cancer cells. Thus side chain on WTMA was deduced to be required for the anti-cancer cell activity. Further study demonstrated that WTMA and all of its hydrolysis products had no inhibition effect on liver LO2 cells. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1300 / 1305

页数：6

共 23 条

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[2]

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