Influenza hemagglutinin mediated fusion of membranes containing poly(ethylene-glycol) grafted lipids: new insights into the fusion mechanism

被引：20

作者：

Chams, V ^{[1
]}

Bonnafous, P ^{[1
]}

Stegmann, T ^{[1
]}

机构：

[1] Inst Pharmacol & Biol Struct, CNRS, UPR 9062, F-31077 Toulouse, France

来源：

FEBS LETTERS | 1999年 / 448卷 / 01期

关键词：

influenza virus; membrane fusion; poly(ethylene-glycol); hemagglutinin;

D O I：

10.1016/S0014-5793(99)00333-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of a hydrophilic layer covering the membrane on influenza hemagglutinin (HA) mediated fusion was investigated using membranes containing poly(ethylene-glycol) grafted phosphatidylethanolamine (PEG-2000-PE). Steric inhibition of HA-membrane interactions by these lipids affected virus fusion (half-maximal inhibition at 0.8 mol% for lipids with 114 ethylene glycol residues, or at 3.2 mol% for 45 residues (PEG-2000-PE), concentrations at which the PEG moieties adopt a random coil structure). Reconstituted viral membranes containing 3 mol% PEG-2000-PE retained 40% of their fusion activity. Therefore, efficient fusion is possible with membranes completely covered by a hydrophilic layer of several nanometers, and fusogenic virosomes containing PEG-PE are feasible. (C) 1999 Federation of European Biochemical Societies.

引用

页码：28 / 32

页数：5

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