Protein disulfide isomerase assists protein folding as both an isomerase and a chaperone

被引：36

作者：

Wang, CC ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

ENZYME ENGINEERING XIV | 1998年 / 864卷

关键词：

D O I：

10.1111/j.1749-6632.1998.tb10283.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protein disulfide isomerase (PDI) is the physiological catalyst of native disulfide bond formation of nascent peptides in the cells. As a foldase, PDI has both isomerase and chaperone activities. The chaperone activity is intrinsic and independent of its isomerase activity. Both chaperone and isomerase activities are required for PDI to assist folding of denatured and reduced disulfide-containing proteins. PDI may have great applications in protein production by bioengineering for its function as a foldase.

引用

页码：9 / 13

页数：5

共 22 条

[1] RENATURATION OF A SINGLE-CHAIN IMMUNOTOXIN FACILITATED BY CHAPERONES AND PROTEIN DISULFIDE ISOMERASE [J].

BUCHNER, J ;

BRINKMANN, U ;

PASTAN, I .

BIO-TECHNOLOGY, 1992, 10 (06) :682-685

[2] Molecular chaperones in protein folding and translocation [J].

Clarke, AR .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1996, 6 (01) :43-50

[3] A mutant truncated protein disulfide isomerase with no chaperone activity [J].

Dai, Y ;

Wang, CC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (44) :27572-27576

[4] Protein folding in the cell: Competing models of chaperonin function [J].

Ellis, RJ ;

Hartl, FU .

FASEB JOURNAL, 1996, 10 (01) :20-26

[5] THE GENERAL CONCEPT OF MOLECULAR CHAPERONES [J].

ELLIS, RJ .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1993, 339 (1289) :257-261

[6] PROTEIN DISULFIDE-ISOMERASE - BUILDING BRIDGES IN PROTEIN-FOLDING [J].

FREEDMAN, RB ;

HIRST, TR ;

TUITE, MF .

TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (08) :331-336

[7] Mutant human protein disulfide isomerase assists protein folding in a chaperone-like fashion [J].

Gao, Y ;

Quan, H ;

Jiang, MY ;

Dai, Y ;

Wang, CC .

JOURNAL OF BIOTECHNOLOGY, 1997, 54 (02) :105-112

[8] PROTEIN FOLDING IN THE CELL [J].

GETHING, MJ ;

SAMBROOK, J .

NATURE, 1992, 355 (6355) :33-45

[9]

GOLDBERGER RF, 1964, J BIOL CHEM, V239, P1406

[10] Co-expression of human protein disulphide isomerase (PDI) can increase the yield of an antibody Fab' fragment expressed in Escherichia coli [J].

Humphreys, DP ;

Weir, N ;

Lawson, A ;

Mountain, A ;

Lund, PA .

FEBS LETTERS, 1996, 380 (1-2) :194-197

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