Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds

被引：1208

作者：

Huangfu, Danwei ^{[1
]}

Maehr, Rene ^{[1
]}

Guo, Wenjun ^{[2
]}

Eijkelenboom, Astrid ^{[1
,3
]}

Snitow, Melinda ^{[1
]}

Chen, Alice E. ^{[1
]}

Melton, Douglas A. ^{[1
]}

机构：

[1] Harvard Univ, Howard Hughes Med Inst, Harvard Stem Cell Inst, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

[2] MIT, Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[3] Univ Utrecht, NL-3508 TC Utrecht, Netherlands

来源：

NATURE BIOTECHNOLOGY | 2008年 / 26卷 / 07期

关键词：

D O I：

10.1038/nbt1418

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase ( HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.

引用

页码：795 / 797

页数：3

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