Simultaneous determination of cyanobacterial hepato- and neurotoxins in water samples by ion-pair supported enrichment and HPLC-ESI-MS-MS

被引：37

作者：

Pietsch, J

Fichtner, S

Imhof, L

Schmidt, W

Brauch, HJ

机构：

[1] DVGW Technol Zentrum Wasser, Aussenstelle Dresden, D-01139 Dresden, Germany

[2] DVGW Technol Zentrum Wasser, D-76139 Karlsruhe, Germany

来源：

CHROMATOGRAPHIA | 2001年 / 54卷 / 5-6期

关键词：

column liquid chromatography; solid phase extraction; MS-MS detection; algal toxins; water samples;

D O I：

10.1007/BF02492680

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyanobacterial neurotoxins, such as anatoxin-a and saxitoxin, as well as hepatotoxins including microcystins and nodularin were simultaneously determined in water samples by ion-pair supported, solid phase extraction (SPE) and reversed-phase liquid chromatography coupled to UV and tandem mass spectrometry (RP-LC-UV; MS-MS). With quantification limits in water samples of approximately 50 ng L-1 for the microcystins (MC-LR, -YR, -RR, -LA), nodularin, and anatoxin-a and 630 ng L-1 for saxitoxin the method is well suited for surveillance of the proposed WHO guidelines for cyanobacterial toxins. MS detection permits, unlike the commonly used UV detection, unambiguous identification and accurate quantification of cyanobacterial toxins even in highly matrix-polluted, water samples.

引用

页码：339 / 344

页数：6

共 38 条

[1] Enzymatic pathway for the bacterial degradation of the cyanobacterial cyclic peptide toxin microcystin LR [J].