Monitoring the growth of a bacteria culture by MALDI-MS of whole cells

被引：131

作者：

Arnold, RJ ^{[1
]}

Karty, JA ^{[1
]}

Ellington, AD ^{[1
]}

Reilly, JP ^{[1
]}

机构：

[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 10期

关键词：

D O I：

10.1021/ac981196c

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

We have probed the time evolution of a growing bacteria culture by extracting samples periodically and performing matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) on whole cells. The mass spectra generated by this method contain tens of peaks in the 3-11-kDa mass range. Cultures of E. coli strain K-12 were grown in two types of containers and at two nutrient concentrations and sampled periodically from 6 to 84 h after inoculation. The relative intensities of several of the stronger peaks vary quite dramatically as a function of time. These temporal characteristics must be taken into account when MALDI-MS is applied to identify bacteria. The results also suggest that MALDI-MS can be used to follow the aging of a bacteria culture.

引用

页码：1990 / 1996

页数：7

共 40 条

[1]

IDENTIFICATION OF BACTERIA USING MASS-SPECTROMETRY [J].