Metabolomic analysis of uremic toxins by liquid chromatography/electrospray ionization-tandem mass spectrometry

被引：69

作者：

Kikuchi, Kaori ^{[2
]}

Itoh, Yoshiharu ^{[2
]}

Tateoka, Ryoko ^{[2
]}

Ezawa, Atsuko ^{[2
]}

Murakami, Kenjiro ^{[2
]}

Niwa, Toshimitsu ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Adv Med Uremia, Showa Ku, Nagoya, Aichi 4668550, Japan

[2] Kureha Corp, Biomed Res Labs, Tokyo, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 20期

关键词：

Liquid chromatography/electrospray; Ionization-tandem mass spectrometry; Principal component analysis; Metabolome; Uremic toxin; Chronic renal failure; Indoxyl sulfate; OSTEOBLAST-SPECIFIC PROTEINS; SMOOTH-MUSCLE-CELLS; INDOXYL SULFATE; OXIDATIVE STRESS; RENAL-FAILURE; P-CRESOL; GLOMERULAR SCLEROSIS; ORAL SORBENT; RAT KIDNEYS; PROGRESSION;

D O I：

10.1016/j.jchromb.2009.11.040

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We applied the metabolomic analysis of comprehensive small-molecular metabolites using liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS)and principal component analysis to identify uremic toxins accumulated in the serum of chronic renal failure (CRF) rats CRF rats were produced by 5/6-nephrectomy Indoxyl sulfate was demonstrated to be the first principal serum metabolite which differentiates CRF from not mal, followed by phenyl sulfate, hippuric acid and p-cresyl sulfate. Then, we measured the serum levels of indoxyl sulfate, phenyl sulfate, hippuric acid and p-cresyl sulfate by the selected reaction monitoring (SRM) of LC/ESI-MS/MS, and demonstrated that these serum levels were markedly increased in CRF rats as compared with normal rats As creatinine clearance decreased, the serum levels of the metabolites increased (C) 2009 Elsevier B V. All rights reserved

引用

页码：1662 / 1668

页数：7

共 23 条

[1]

Indoxyl sulphate promotes aortic calcification with expression of osteoblast-specific proteins in hypertensive rats [J].

Adijiang, Ayinuer ;

Goto, Sumie ;

Uramoto, Satsuki ;

Nishijima, Fuyuhiko ;

Niwa, Toshimitsu .

NEPHROLOGY DIALYSIS TRANSPLANTATION, 2008, 23 (06) :1892-1901

[2]

Combination therapy with benazepril and oral adsorbent ameliorates progressive renal fibrosis in uremic rats [J].

Aoyama, I ;

Shimokata, K ;

Niwa, T .

NEPHRON, 2002, 90 (03) :297-312

[3]

Gas chromatographic-mass spectrometric analysis for measurement of p-cresol and its conjugated metabolites in uremic and normal serum [J].

de Loor, H ;

Bammens, B ;

Evenepoel, P ;

De Preter, V ;

Verbeke, K .

CLINICAL CHEMISTRY, 2005, 51 (08) :1535-1538

[4]

Removal of p-cresol sulfate by hemodialysis [J].

Martinez, AW ;

Recht, NS ;

Hostetter, TH ;

Meyer, TW .

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2005, 16 (11) :3430-3436

[5]

Miyazaki T, 1997, KIDNEY INT, V52, pS15

[6]

An oral sorbent reduces overload of indoxyl sulphate and gene expression of TGF-β1 in uraemic rat kidneys [J].

Miyazaki, T ;

Aoyama, I ;

Ise, M ;

Seo, H ;

Niwa, T .

NEPHROLOGY DIALYSIS TRANSPLANTATION, 2000, 15 (11) :1773-1781

[7]

Indoxyl Sulfate Promotes Proliferation of Human Aortic Smooth Muscle Cells by Inducing Oxidative Stress [J].

Muteliefu, Gulinuer ;

Enomoto, Atsushi ;

Niwa, Toshimitsu .

JOURNAL OF RENAL NUTRITION, 2009, 19 (01) :29-32

[8]

Indoxyl sulphate induces oxidative stress and the expression of osteoblast-specific proteins in vascular smooth muscle cells [J].

Muteliefu, Gulinuer ;

Enomoto, Atsushi ;

Jiang, Ping ;

Takahashi, Masahide ;

Niwa, Toshimitsu .

NEPHROLOGY DIALYSIS TRANSPLANTATION, 2009, 24 (07) :2051-2058

[9]

Niwa T, 1997, MINER ELECTROL METAB, V23, P179

[10]

Niwa T, 1997, KIDNEY INT, V52, pS23

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