Development of a novel screen for protease inhibitors

被引：3

作者：

Gillim, L

Gusella, GL

Vargas, J

Marras, D

Klotman, ME

Cara, A

机构：

[1] CUNY Mt Sinai Sch Med, Div Infect Dis, New York, NY 10029 USA

[2] CUNY Mt Sinai Sch Med, Div Nephrol, New York, NY 10029 USA

[3] Ist Super Sanita, I-00161 Rome, Italy

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2001年 / 8卷 / 02期

关键词：

D O I：

10.1128/CDLI.8.2.437-440.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have developed a novel plasmid-based, quantitative, in vitro screen to test the protease-inhibiting activities of existing and newly discovered agents.

引用

页码：437 / 440

页数：4

共 9 条

[1] POTENT INHIBITION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) REPLICATION BY INDUCIBLE EXPRESSION OF HIV-1 PR MULTIMERS [J].

ARRIGO, SJ ;

HUFFMAN, K .

JOURNAL OF VIROLOGY, 1995, 69 (10) :5988-5994

[2] Viral proteases: Evolution of diverse structural motifs to optimize function [J].

Babe, LM ;

Craik, CS .

CELL, 1997, 91 (04) :427-430

[3] Exploration of the Drosophila acetylcholinesterase substrate activation site using a reversible inhibitor (Triton X-100) and mutated enzymes [J].

Marcel, V ;

Estrada-Mondaca, S ;

Magné, F ;

Stojan, J ;

Klaébé, A ;

Fournier, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (16) :11603-11609

[4] An Escherichia coli expression assay and screen for human immunodeficiency virus protease variants with decreased susceptibility to indinavir [J].

Melnick, L ;

Yang, SS ;

Rossi, R ;

Zepp, C ;

Heefner, D .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (12) :3256-3265

[5] Human serum attenuates the activity of protease inhibitors toward wild-type and mutant human immunodeficiency virus [J].

Molla, A ;

Vasavanonda, S ;

Kumar, G ;

Sham, HL ;

Johnson, M ;

Grabowski, B ;

Denissen, JF ;

Kohlbrenner, W ;

Plattner, JJ ;

Leonard, JM ;

Norbeck, DW ;

Kempf, DJ .

VIROLOGY, 1998, 250 (02) :255-262

[6] Use of GFP as a reporter for the facile analysis of sequence-specific proteases [J].

Pehrson, JC ;

Weatherman, A ;

Markwell, J ;

Sarath, G ;

Schwartzbach, SD .

BIOTECHNIQUES, 1999, 27 (01) :28-+

[7] Epitope-based assay to determine the efficiency of cleavage by HIV-1 protease [J].

Serio, D ;

Weber, IT ;

Harrison, RW ;

Louis, JM ;

Srinivasan, A .

BIOTECHNIQUES, 1999, 26 (02) :242-+

[8] Development of in vitro peptide substrates for human rhinovirus-14 2A protease [J].

Wang, QM ;

Johnson, RB ;

Sommergruber, W ;

Shepherd, TA .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 356 (01) :12-18

[9]

Yamamoto T, 1998, BIOL PHARM BULL, V21, P1148

← 1 →