Focal ischemia induces expression of the DNA damage-inducible gene GADD45 in the rat brain

被引：56

作者：

Jin, KL ^{[1
]}

Chen, J ^{[1
]}

Kawaguchi, K ^{[1
]}

Zhu, RL ^{[1
]}

Stetler, RA ^{[1
]}

Simon, RP ^{[1
]}

Graham, SH ^{[1
]}

机构：

[1] UNIV PITTSBURGH,SCH MED,DEPT NEUROL,PITTSBURGH,PA 15261

来源：

NEUROREPORT | 1996年 / 7卷 / 11期

关键词：

GADD45; cerebral ischemia and reperfusion; DNA damage; DNA repair; gene;

D O I：

10.1097/00001756-199607290-00022

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

GADD45 is a DNA damage-inducible gene that accelerates DNA excision repair and can be induced by a variety of DNA-damaging stimuli in mammalian cells. We investigated the expression of GADD45 mRNA and protein using in situ hybridization and immunocytochemistry in rat brains after 2 h of temporal focal ischemia. The expression of GADD45 mRNA was induced in neurons throughout ischemic cortex 4 h after the onset of ischemia but was restricted to ischemic penumbra regions at 24 h after ischemia. The expression of GADD45 protein was increased only in sublethally injured neurons in the penumbra regions at both 4 h and 24 h following ischemia. These results suggest that GADD45 could have a protective role in ischemic neurons.

引用

页码：1797 / 1802

页数：6

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