Calcium flux in neutrophils synchronizes β2 integrin adhesive and signaling events that guide inflammatory recruitment

被引：78

作者：

Schaff, Ulrich Y. ^{[1
]}

Yamayoshi, Itsukyo ^{[1
]}

Tse, Tiffany ^{[1
]}

Griffin, Donald ^{[1
]}

Kibathi, Lilian ^{[1
]}

Simon, Scott I. ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Biomed Engn, Genome & Biomed Sci Facil, Davis, CA 95616 USA

来源：

ANNALS OF BIOMEDICAL ENGINEERING | 2008年 / 36卷 / 04期

关键词：

neutrophils; shear stress; calcium signaling; integrin affinity;

D O I：

10.1007/s10439-008-9453-8

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Intracellular calcium flux is an early step in the signaling cascade that bridges ligation of selectin and chemokine receptors to activation of adhesive and motile functions during recruitment on inflamed endothelium. Calcium flux was imaged in real time and provided a means of correlating signaling events in neutrophils rolling on E-selectin and stimulated by chemokine in a microfluidic chamber. Integrin dependent neutrophil arrest was triggered by E-selectin tethering and ligation of IL-8 seconds before a rapid rise in intracellular calcium, which was followed by the onset of pseudopod formation. Calcium flux on rolling neutrophils increased in a shear dependent manner, and served to link integrin adhesion and signaling of cytoskeletally driven cell polarization. Abolishing calcium influx through membrane expressed store operated calcium channels inhibited activation of high affinity beta(2) integrin and subsequent cell arrest. We conclude that calcium influx at the plasma membrane integrates chemotactic and adhesive signals, and functions to synchronize signaling of neutrophil arrest and migration in a shear stress dependent manner.

引用

页码：632 / 646

页数：15

共 52 条

[1] Chemokine induction of integrin adhesiveness on rolling and arrested leukocytes local signaling events or global stepwise activation? [J].

Alon, R ;

Grabovsky, V ;

Feigelson, S .

MICROCIRCULATION, 2003, 10 (3-4) :297-311

[2] From rolling to arrest on blood vessels: leukocyte tap dancing on endothelial integrin ligands and chemokines at sub-second contacts [J].

Alon, R ;

Feigelson, S .

SEMINARS IN IMMUNOLOGY, 2002, 14 (02) :93-104

[3] Force as a facilitator of integrin conformational changes during leukocyte arrest on blood vessels and antigen-presenting cells [J].

Alon, Ronen ;

Dustin, Michael L. .

IMMUNITY, 2007, 26 (01) :17-27

[4] Chemoattractant signals and β2 integrin occupancy at apical endothelial contacts combine with shear stress signals to promote transendothelial neutrophil migration [J].

Cinamon, G ;

Shinder, V ;

Shamri, R ;

Alon, R .

JOURNAL OF IMMUNOLOGY, 2004, 173 (12) :7282-7291

[5] High micromolar Ca2+ beneath the plasma membrane in stimulated neutrophils [J].

Davies, EV ;

Hallett, MB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 248 (03) :679-683

[6] A ROLE FOR CALCIUM AND PROTEIN KINASE-C IN AGONIST-STIMULATED ADHESION OF HUMAN NEUTROPHILS [J].

DAVIES, MPA ;

HALLAM, TJ ;

MERRITT, JE .

BIOCHEMICAL JOURNAL, 1990, 267 (01) :13-16

[7] Immobilized IL-8 triggers progressive activation of neutrophils rolling in vitro on P-selectin and intercellular adhesion molecule-1 [J].

DiVietro, JA ;

Smith, MJ ;

Smith, BRE ;

Petruzzelli, L ;

Larson, RS ;

Lawrence, MB .

JOURNAL OF IMMUNOLOGY, 2001, 167 (07) :4017-4025

[8] CHEMOTACTIC FACTOR-RECEPTOR ACTIVATION TRANSIENTLY IMPAIRS THE CA2+ SIGNALING CAPACITY OF BETA(2) INTEGRINS ON HUMAN NEUTROPHILS [J].

EIERMAN, D ;

HELLBERG, C ;

SJOLANDER, A ;

ANDERSSON, T .

EXPERIMENTAL CELL RESEARCH, 1994, 215 (01) :90-96

[9]

Elzi DJ, 2001, AM J PHYSIOL-CELL PH, V281, pC350

[10] A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function [J].

Feske, S ;

Gwack, Y ;

Prakriya, M ;

Srikanth, S ;

Puppel, SH ;

Tanasa, B ;

Hogan, PG ;

Lewis, RS ;

Daly, M ;

Rao, A .

NATURE, 2006, 441 (7090) :179-185

← 1 2 3 4 5 6 →