Clinical validation of a real-time polymerase chain reaction detection of Neisseria gonorrheae porA pseudogene versus culture techniques

被引:18
作者
Hjelmevoll, Stig Ove [1 ]
Olsen, Merethe Elise [1 ]
Sollid, Johanna U. Ericson [2 ]
Haaheim, Hakon [1 ]
Melby, Kjetil K. [3 ]
Moi, Harald [4 ]
Unemo, Magnus [5 ]
Skogen, Vegard [6 ,7 ]
机构
[1] Univ Hosp N Norway, Dept Microbiol & Infect Control, N-9038 Tromso, Norway
[2] Univ Tromso, Inst Med Biol, Dept Microbiol & Virol, Tromso, Norway
[3] Ullevaal Univ Hosp, Dept Microbiol, Oslo, Norway
[4] Univ Oslo, Olafiaklin, N-0316 Oslo, Norway
[5] Orebro Univ Hosp, Natl Reference Lab Pathogen Neisseria, Dept Clin Microbiol, Orebro, Sweden
[6] Univ Hosp N Norway, Dept Internal Med B, Tromso, Norway
[7] Univ Tromso, Inst Clin Med, Tromso, Norway
关键词
D O I
10.1097/OLQ.0b013e3181644bc9
中图分类号
R51 [传染病];
学科分类号
100401 [流行病与卫生统计学];
摘要
Background: Diagnosing Neisseria gonorrheae using nucleic acid amplification tests (NAATs) might increase the sensitivity, compared to cultivation. However, using NAATs has also been problematic mainly due to the close genetic relationships between different Neisseria species, resulting in false positive diagnoses. This study was conducted to clinically validate a previously published real-time polymerase chain reaction (PCR) method targeting the porA pseudogene in N. gonorrheae in comparison to culture techniques. Methods: In total, 360 samples, urethra (n = 109), rectum (n = 84), pharynx (n = 119), and cervix (n = 48) from 185 males and 57 females, were analyzed using porA pseudogene PCR and cultivation. Sequencing of the entire porA pseudogene and the 16S rRNA gene were used to resolve discrepant results. Results: Of the 360 samples, 37 were positive by both culture and PCR, however, the PCR identified 15 additional confirmed positive samples. The PCR method showed a sensitivity, specificity, positive predictive value, and negative predictive value of 100% in a preselected population. The preselected population had a true gonorrhea prevalence of 17.4%. Conclusions: The present porA pseudogene real-time PCR comprises a valuable supplement to the traditional culture techniques for diagnosis of N. gonorrheae, especially for samples from extragenital sites such as pharynx and rectum.
引用
收藏
页码:517 / 520
页数:4
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