The effects of various extracellular matrices on renal cell attachment to polymer surfaces during the development of bioartificial renal tubules

Extracellular matrices (ECM) are utilized for obtaining better cell attachment to polymer surfaces in cell cultures. To establish beneficial bioartificial renal tubules, tubular epithelial cells and ECM were investigated in this study. MDCK cells and KU-2 cells were seeded onto 96 well plates which had been precoated with collagen types I and IV, laminin, and fibronectin. The culture media were removed and replaced with new ones at 15, 30, 60, 90, 120, and 150 min and 24 h after start time to evaluate the incubation Lime effects. The degrees of cell attachment onto ECM were measured by MTT assay. In the MDCK cell culture, better cell attachment was observed between 60 min and 24 h after incubation start time with the use of laminin at a concentration of 5 mu g/ml, 60 min and more after incubation start time with the use of fibronectin at the concentrations of 1 and 4 mu g/ml, or 30 min and more after incubation start time with the use of fibronectin at the concentrations of 16 and 32 mu g/ml. On the other hand, in the KU-2 cell culture, better cell attachment was observed between 15 and 60 min after the incubation start time or 24 h after the incubation start time with the use of laminin at a concentration of 40 mu g/ml. These data suggest that various cells possibly each have a most suitable ECM kind, best concentration, and best incubation time.