Rapid generation of a human monoclonal antibody to combat Middle East respiratory syndrome

被引：29

作者：

Corti, Davide ^{[1
]}

Passini, Nadia ^{[1
]}

Lanzavecchia, Antonio ^{[2
,3
]}

Zambon, Maria ^{[4
]}

机构：

[1] Humabs BioMed SA, CH-6500 Bellinzona, Switzerland

[2] Univ Svizzera Italiana, Inst Biomed Res, Immune Regulat Unit, CH-6500 Bellinzona, Switzerland

[3] ETH, Inst Microbiol, CH-8093 Zurich, Switzerland

[4] Publ Hlth England, Microbiol Serv Colindale, London NW9 5HT, England

来源：

JOURNAL OF INFECTION AND PUBLIC HEALTH | 2016年 / 9卷 / 03期

关键词：

SYNDROME CORONAVIRUS;

D O I：

10.1016/j.jiph.2016.04.003

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The last century has witnessed the emergence of several previously unknown viruses as life-threatening human pathogens. Several examples include HIV, Ebola, Lujo, and, most recently, the Middle East respiratory syndrome (MERS) and Ebola. In this study, we describe a method for the swift generation of a human derived monoclonal antibody, known as LCA60, as a treatment for MERS infections. LCA60 antibody was generated using the Cellclone Technology from the immortalized B cells of a human donor recovering from MERS. Only four months were required from the initial screening of B cells to the development of a stable CHO cell line suitable for the production of clinical grade antibody, thereby delineating a rapid pathway for the development of antiviral therapies against emerging viruses. Currently, the LCA60 antibody is being considered for clinical development, which includes prophylaxis in individuals at risk and a treatment for severe MERS-CoV infections. (C) 2016 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Limited. All rights reserved.

引用

页码：231 / 235

页数：5

共 11 条

[1] Clinical Course and Outcomes of Critically Ill Patients With Middle East Respiratory Syndrome Coronavirus Infection [J].

Arabi, Yaseen M. ;

Arifi, Ahmed A. ;

Balkhy, Hanan H. ;

Najm, Hani ;

Aldawood, Abdulaziz S. ;

Ghabashi, Alaa ;

Hawa, Hassan ;

Alothman, Adel ;

Khaldi, Abdulaziz ;

Al Raiy, Basel .

ANNALS OF INTERNAL MEDICINE, 2014, 160 (06) :389-+

[2]

Assiri A, 2013, N ENGL J MED

[3]

Bermingham A, 2012, EUROSURVEILLANCE, V17, P6

[4] Combating Emerging Threats - Accelerating the Availability of Medical Therapies [J].

Borio, Luciana ;

Cox, Edward ;

Lurie, Nicole .

NEW ENGLAND JOURNAL OF MEDICINE, 2015, 373 (11) :993-995

[5] Swift antibodies to counter emerging viruses [J].

Burton, Dennis R. ;

Saphire, Erica Ollmann .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (33) :10082-10083

[6] Efficient Methods To Isolate Human Monoclonal Antibodies from Memory B Cells and Plasma Cells [J].

Corti, Davide ;

Lanzavecchia, Antonio .

MICROBIOLOGY SPECTRUM, 2014, 2 (05)

[7] Prophylactic and postexposure efficacy of a potent human monoclonal antibody against MERS coronavirus [J].

Corti, Davide ;

Zhao, Jincun ;

Pedotti, Mattia ;

Simonelli, Luca ;

Agnihothram, Sudhakar ;

Fett, Craig ;

Fernandez-Rodriguez, Blanca ;

Foglierini, Mathilde ;

Agatic, Gloria ;

Vanzetta, Fabrizia ;

Gopal, Robin ;

Langrish, Christopher J. ;

Barrettg, Nicholas A. ;

Sallusto, Federica ;

Baric, Ralph S. ;

Varani, Luca ;

Zambon, Maria ;

Perlman, Stanley ;

Lanzavecchia, Antonio .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (33) :10473-10478

[8] Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation [J].

Haagmans, Bart L. ;

Al Dhahiry, Said H. S. ;

Reusken, Chantal B. E. M. ;

Raj, V. Stalin ;

Galiano, Monica ;

Myers, Richard ;

Godeke, Gert-Jan ;

Jonges, Marcel ;

Farag, Elmoubasher ;

Diab, Ayman ;

Ghobashy, Hazem ;

Alhajri, Farhoud ;

Al-Thani, Mohamed ;

Al-Marri, Salih A. ;

Al Romaihi, Hamad E. ;

Al Khal, Abdullatif ;

Bermingham, Alison ;

Osterhaus, Albert D. M. E. ;

AlHajri, Mohd M. ;

Koopmans, Marion P. G. .

LANCET INFECTIOUS DISEASES, 2014, 14 (02) :140-145

[9] The Effectiveness of Convalescent Plasma and Hyperimmune Immunoglobulin for the Treatment of Severe Acute Respiratory Infections of Viral Etiology: A Systematic Review and Exploratory Meta-analysis [J].

Mair-Jenkins, John ;

Saavedra-Campos, Maria ;

Baillie, J. Kenneth ;

Cleary, Paul ;

Khaw, Fu-Meng ;

Lim, Wei Shen ;

Makki, Sophia ;

Rooney, Kevin D. ;

Nguyen-Van-Tam, Jonathan S. ;

Beck, Charles R. .

JOURNAL OF INFECTIOUS DISEASES, 2015, 211 (01) :80-90

[10]

Traggiai E, 2004, NAT MED, V10, P871, DOI 10.1038/nm1080

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