A flash-annealing technique to improve diffraction limits and lower mosaicity in crystals of glycerol kinase

被引：83

作者：

Yeh, JI

Hol, WGJ

机构：

[1] Univ Washington, Dept Biol Struct, Biomol Struct Ctr, Seattle, WA 98195 USA

[2] Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1998年 / 54卷

关键词：

D O I：

10.1107/S0907444998004697

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A flash-annealing method has been developed that increases the diffraction limits, and simultaneously decreases the mosaicity of glycerol kinase crystals. This technique utilizes brief thawing and rapid freezing cycles of the crystal in the cold nitrogen stream. The effective resolution limits increased almost by 0.8 Angstrom, from 3.6 to 2.8 Angstrom, and mosaicity values halved.

引用

页码：479 / 480

页数：2

共 5 条

[1] Cloning and sequencing of two Enterococcal glpK genes and regulation of the encoded glycerol kinases by phosphoenolpyruvate dependent, phosphotransferase system-catalyzed phosphorylation of a single histidyl residue [J].