Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination

被引：162

作者：

Lorenzen, N ^{[1
]}

Lorenzen, E ^{[1
]}

Einer-Jensen, K ^{[1
]}

Heppell, J ^{[1
]}

Wu, T ^{[1
]}

Davis, H ^{[1
]}

机构：

[1] Danish Vet Lab, Aarhus 8200 N, Denmark

来源：

FISH & SHELLFISH IMMUNOLOGY | 1998年 / 8卷 / 04期

关键词：

DNA vaccine; Engtved virus; antibody response; protection;

D O I：

10.1006/fsim.1997.0134

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Rainbow trout fingerlings were immunized by intramuscular injection of a plasmid DNA vector encoding the viral haemorrhagic septicaemia virus (VHSV) glycoprotein (G) or nucleocapsid protein (N) genes under the control of a cytomegalovirus promoter. Challenge with VHSV 52 days later demonstrated that both viral genes, and the G gene in particular, were able to induce protective immunity against VHS. In contrast to sera taken from fish injected with the N gene, neutralizing antibody activity could be detected both before and after challenge in the sera of a major proportion of the fish injected with the G gene. (C) 1998 Academic Press Limited.

引用

页码：261 / 270

页数：10

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