Effect of free radical scavengers on diaphragmatic contractility in septic peritonitis

被引：56

作者：

Fujimura, N ^{[1
]}

Sumita, S ^{[1
]}

Aimono, M ^{[1
]}

Masuda, Y ^{[1
]}

Shichinohe, Y ^{[1
]}

Narimatsu, E ^{[1
]}

Namiki, A ^{[1
]}

机构：

[1] Sapporo Med Univ, Sch Med, Dept Anesthesiol, Chuo Ku, Sapporo, Hokkaido 0600061, Japan

来源：

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | 2000年 / 162卷 / 06期

关键词：

D O I：

10.1164/ajrccm.162.6.9912144

中图分类号：

R4 [临床医学];

学科分类号：

1002 [临床医学]; 100602 [中西医结合临床];

摘要：

We investigated the effects of polyethylene glycol-adsorbed superoxide dismutase (PEG-SOD), polyethylene glycol-adsorbed catalase (PEC-CAT), and DMSO on diaphragmatic contractility and malondialdehyde (MDA) concentrations in septic peritonitis in vitro. One hundred eighty-six rats were divided into two groups. One group (CLP group) was treated with cecal ligation and perforation (CLP), and the other (sham group) was treated with laparotomy. PEG-SOD, PEG-CAT, and DMSO were administered intraperitoneally 30 min before and 12 h after CLP. The left hemidiaphragm was removed at 10 h or 16 h after the operation. We assessed the diaphragmatic contractility by twitch characteristics and force-frequency curves in vitro. We measured MDA concentrations, as an index of oxygen-derived free radical-mediated lipid peroxidation, and the activities of two main antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx), as an index of antioxidant defenses, after CLP. Diaphragmatic force generation capacity was significantly reduced after CLP. Diaphragmatic: MDA levels were significantly elevated after CLP. PEC-SOD, PEG-CAT, and DMSO significantly improved diaphragmatic contractility and prevented the elevation in diaphragmatic MDA concentrations after CLP. Diaphragmatic SOD activities were significantly increased after CLP. These results suggest that several types of oxygen-derived free radicals play a role in the reduction in diaphragmatic contractility after CLP.

引用

页码：2159 / 2165

页数：7

共 33 条

[1]

SINGLE-DOSE TUMOR-NECROSIS-FACTOR PROTECTION AGAINST ENDOTOXIN-INDUCED SHOCK AND TISSUE-INJURY IN RATS [J].

ALEXANDER, HR ;

DOHERTY, GM ;

BLOCK, MI ;

KRAGEL, PJ ;

JENSEN, JC ;

LANGSTEIN, HN ;

WALKER, E ;

NORTON, JA .

INFECTION AND IMMUNITY, 1991, 59 (11) :3889-3894

[2]

MECHANISMS OF DISEASE - ARE OXYGEN-DERIVED FREE-RADICALS INVOLVED IN DIAPHRAGMATIC DYSFUNCTION [J].

ANZUETO, A ;

SUPINSKI, GS ;

LEVINE, SM ;

JENKINSON, SG .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1994, 149 (04) :1048-1052

[3]

BAKER CC, 1985, SURG FORUM, V36, P68

[4]

Lipopolysaccharide signal transduction, regulation of tumor necrosis factor biosynthesis, and signaling by tumor necrosis factor itself [J].

Beutler, B ;

Kruys, V .

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1995, 25 :S1-S8

[5]

Endogenous peroxynitrite mediates mitochondrial dysfunction in rat diaphragm during endotoxemia [J].

Boczkowski, J ;

Lisdero, CL ;

Lanone, S ;

Samb, A ;

Carreras, MC ;

Boveris, A ;

Aubier, M ;

Poderoso, JJ .

FASEB JOURNAL, 1999, 13 (12) :1637-1646

[6]

EFFECTS OF SEPSIS ON DIAPHRAGMATIC FUNCTION IN RATS [J].

BOCZKOWSKI, J ;

DUREUIL, B ;

BRANGER, C ;

PAVLOVIC, D ;

MURCIANO, D ;

PARIENTE, R ;

AUBIER, M .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1988, 138 (02) :260-265

[7]

CHEMISTRY AND BIOCHEMISTRY OF 4-HYDROXYNONENAL, MALONALDEHYDE AND RELATED ALDEHYDES [J].

ESTERBAUER, H ;

SCHAUR, RJ ;

ZOLLNER, H .

FREE RADICAL BIOLOGY AND MEDICINE, 1991, 11 (01) :81-128

[8]

LABORATORY MODELS OF SEPSIS AND SEPTIC SHOCK [J].

FINK, MP ;

HEARD, SO .

JOURNAL OF SURGICAL RESEARCH, 1990, 49 (02) :186-196

[9]

Diaphragmatic contractility in septic peritonitis - Role of free radical scavengers [J].

Fujimura, N ;

Sumita, S ;

Narimatsu, E ;

Shichinohe, Y ;

Iwasaki, H ;

Watanabe, H ;

Namiki, A .

CRITICAL CARE MEDICINE, 1999, 27 (01) :A41-A41

[10]

FREE-RADICALS AND ANTIOXIDANTS IN SEPSIS [J].

GOODE, HF ;

WEBSTER, NR .

CRITICAL CARE MEDICINE, 1993, 21 (11) :1770-1776

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