In vitro and in vivo study of N-trimethyl chitosan nanoparticles for oral protein delivery

被引：114

作者：

Chen, Fu ^{[1
]}

Zhang, Zhi-Rong ^{[1
]}

Yuan, Fang ^{[1
]}

Qin, Xuan ^{[1
]}

Wang, Minting ^{[1
]}

Huang, Yuan ^{[1
]}

机构：

[1] Sichuan Univ, W China Sch Pharm, Minist Educ, Kay Lab Drug Targeting, Chengdu 610041, Peoples R China

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2008年 / 349卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

N-trimethyl chitosan; nanoparticles; protein carriers; oral vaccination;

D O I：

10.1016/j.ijpharm.2007.07.035

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In this study, the effects of alginate modification on absorption properties of FITC-BSA loaded TMC nanoparticles were investigated on an in vitro model of GI epithelium (Caco-2 cells). The feasibility of applying TMC nanoparticles loaded with a model vaccine urease in oral vaccination was also studied. Alginate modified TMC nanoparticles showed higher FITC-BSA permeate efficiency than non-modified TMC nanoparticles. However, alginate modification barely had any effect on TMC nanoparticles' property of decreasing TEER or enhancing drug paracellular transport. Mice s.c. immunized with urease loaded TMC nanoparticles showed highest systematic immune response (IgG levels) but the lowest mucosal response (secretory IgA levels). In the contrast, mice i.g. immunized with urease loaded TMC nanoparticles showed much higher antibody titers of both IgG and secretory IgA than those with urease solution or urease co-administrated with TMC solution. These results indicated that TMC nanoparticles are potential carriers for oral protein and vaccine delivery. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：226 / 233

页数：8

共 51 条

[1] Preparation and characterization of protein-loaded N-trimethyl chitosan nanoparticles as nasal delivery system [J].