Atomic Force Microscopy Study of Ganglioside GM1 Concentration Effect on Lateral Phase Separation of Sphingomyelin/Dioleoylphosphatidylcholine/Cholesterol Bilayers

被引：27

作者：

Bao, Ren ^{[1
]}

Li, Li ^{[1
]}

Qiu, Feng ^{[1
]}

Yang, Yuliang ^{[1
]}

机构：

[1] Fudan Univ, Key Lab Mol Engn Polymers, Minist Educ, Dept Macromol Sci,Ctr Anal & Measurement, Shanghai 200433, Peoples R China

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2011年 / 115卷 / 19期

基金：

国家高技术研究发展计划(863计划);

关键词：

SUPPORTED LIPID-BILAYERS; MEMBRANES; RAFTS; CHOLESTEROL; DOMAINS; AGGREGATION; MONOLAYERS; G(M1)/DIPALMITOYLPHOSPHATIDYLCHOLINE/DIOLEOYLPHOSPHATIDYLCHOLINE; PHOSPHATIDYLCHOLINE; ORGANIZATION;

D O I：

10.1021/jp2008122

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070305 [高分子化学与物理];

摘要：

The effect of monosialoganglioside GM1 (GM1) concentration on the lateral phase separation in the sphingomyelin/1,2-dioleoyl-sn-glycero-3-phosphocholine/cholesterol (SM/DOPC/Chol) bilayers was studied by using atomic force microscopy. The results show that, with the increase of GM1 mol fraction (x), the dominant composition of liquid-ordered (L(o)) domains changes from SM to SM/GM1 and finally to GM1. Meanwhile, the decrease of domain area (A) of the L(o) phase with the increase of x follows a scaling law of A similar to x (-3/2), for x > 0.005, indicating that the domain growth is pinned with high GM1 concentration. Results of in situ experiments of GM1 insertion into SM/DOPC/cholesterol bilayers further supported our observations.

引用

页码：5923 / 5929

页数：7

共 32 条

[1]

CALORIMETRIC STUDIES ON VARIOUS GANGLIOSIDES AND GANGLIOSIDE-LIPID INTERACTIONS [J].