Development and characterization of polymorphic microsatellites from Prunus avium 'Napoleon'

被引：85

作者：

Clarke, JB ^{[1
]}

Tobutt, KR ^{[1
]}

机构：

[1] Hort Res Int, Maidstone ME19 6BJ, Kent, England

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 04期

关键词：

microsatellite; Prunus avium; simple sequence repeat; sweet cherry;

D O I：

10.1046/j.1471-8286.2003.00517.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Primers were developed for 21 microsatellite loci isolated by enrichment from Prunus avium 'Napoleon'. Twelve loci contained uninterrupted dinucleotide repeats and nine were more complex. Nineteen primer pairs (EMPA001-019) showed single locus polymorphisms in a cultivar survey of 14 sweet cherries, with two to seven alleles per locus. Three primer pairs in combination (EMPA014, 015 and 018) discriminated all cultivars. Two primer pairs for loci monomorphic in P. avium were included: EMPA020 revealed segregation in an interspecific progeny and EMPA021 revealed polymorphism in P. dulcis. Twelve primer pairs reliably amplified products in three peach cultivars of which seven revealed polymorphisms.

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收藏

页码：578 / 580

页数：3

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