PCR-genosensor rapid test for detecting Salmonella

被引:25
作者
Pividori, MI
Merkoçi, A
Barbé, J
Alegret, S [1 ]
机构
[1] Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra 08193, Catalonia, Spain
[2] Univ Autonoma Barcelona, Dept Genet & Microbiol, Unitat Microbiol, Bellaterra 08193, Catalonia, Spain
[3] Univ Nacl Litoral, Fac Bioquim, RA-3000 Santa Fe, Argentina
关键词
graphite-epoxy composite; DNA; hybridization; enzyme labeling; nylon membrane; amperometric genosensors; DNA biosensor; Salmonella spp; IS200;
D O I
10.1002/elan.200302764
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A PCR-genosensor based assay for detecting the IS200 DNA sequence specific to Salmonella spp is reported. This rapid test is based on PCR, where amplicon detection is achieved electrochemically using GEC (graphite-epoxy composite) genosensor. The amplicon is immobilized onto GEC electrodes by simple dry-adsorption and the detection is carried out using an enzymatic labeling system. Results demonstrate that this new electrochemical genosensor fulfils the requirements desired for these devices: easy preparation - as dry-adsorption of DNA is very simple -, robustness, sensitivity, low cost, simple use and fast response. Additionally, the assay can be produced as a kit format. increasing its commercial potentiality. Also, the electrode material can be implemented for screen-printing procedures for the mass production of genosensors.
引用
收藏
页码:1815 / 1823
页数:9
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