Antioxidant effect of naturally occurring xanthines on the oxidative damage of DNA bases

被引：11

作者：

Vieira, AJSC

Telo, JP

Pereira, HF

Patrocínio, PF

Dias, RMB

机构：

[1] Univ Tecn Lisboa, Seccao Quim Organ, Inst Super Tecn, P-1096 Lisbon, Portugal

[2] Inst Tecnol & Nucl, Dept Quim, P-2686 Sacavem, Portugal

来源：

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE | 1999年 / 96卷 / 01期

关键词：

antioxidants; DNA damage repair; oxidation potential; oxidised DNA bases; xanthines;

D O I：

10.1051/jcp:1999118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The repair of the oxidised radicals of adenine and guanosine by several naturally occurring xanthines was studied. Each pair of DNA purine/xanthine was made to react with the sulphate radical and the decrease of the concentration of both compounds was measured by HPLC as a function of irradiation time. The results show that xanthine efficiently prevents the oxidation of the two DNA purines, Theophyline and paraxanthine repair the oxidised radical of adenine but not the one from guanosine. Theobromine and caffeine do not show any protecting effect. An order of the oxidation potentials of all the purines studied is proposed.

引用

收藏

页码：116 / 123

页数：8

相关论文

共 10 条

[1] URIC-ACID PROVIDES AN ANTIOXIDANT DEFENSE IN HUMANS AGAINST OXIDANT-CAUSED AND RADICAL-CAUSED AGING AND CANCER - A HYPOTHESIS [J].

AMES, BN ;

CATHCART, R ;

SCHWIERS, E ;

HOCHSTEIN, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11) :6858-6862

[2] ANTIOXIDATION MECHANISMS OF URIC-ACID [J].

SIMIC, MG ;

JOVANOVIC, SV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (15) :5778-5782

[3]

SOSLER HA, 1968, HDB BIOCH

[4] PURINE-BASES, NUCLEOSIDES, AND NUCLEOTIDES - AQUEOUS-SOLUTION REDOX CHEMISTRY AND TRANSFORMATION REACTIONS OF THEIR RADICAL CATIONS AND E- AND OH ADDUCTS [J].

STEENKEN, S .

CHEMICAL REVIEWS, 1989, 89 (03) :503-520

[5] How easily oxidizable is DNA? One-electron reduction potentials of adenosine and guanosine radicals in aqueous solution [J].

Steenken, S ;

Jovanovic, SV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (03) :617-618

[6] Mechanism of free radical oxidation of caffeine in aqueous solution [J].

Telo, JP ;

Vieira, AJSC .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1997, (09) :1755-1757

[7] PATTERN OF OH RADICAL REACTION WITH 6-SUBSTITUTED AND 9-SUBSTITUTED PURINES - EFFECT OF SUBSTITUENTS ON THE RATES AND ACTIVATION PARAMETERS OF THE UNIMOLECULAR TRANSFORMATION REACTIONS OF 2 ISOMERIC OH ADDUCTS [J].

VIEIRA, AJSC ;

STEENKEN, S .

JOURNAL OF PHYSICAL CHEMISTRY, 1987, 91 (15) :4138-4144

[8] PATTERN OF OH RADICAL REACTION WITH ADENINE AND ITS NUCLEOSIDES AND NUCLEOTIDES - CHARACTERIZATION OF 2 TYPES OF ISOMERIC OH ADDUCT AND THEIR UNIMOLECULAR TRANSFORMATION REACTIONS [J].

VIEIRA, AJSC ;

STEENKEN, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (19) :6986-6994

[9] Absorption spectra of isomeric OH adducts of 1,3,7-trimethylxanthine [J].

Vinchurkar, MS ;

Rao, BSM ;

Mohan, H ;

Mittal, JP ;

Schmidt, KH ;

Jonah, CD .

JOURNAL OF PHYSICAL CHEMISTRY A, 1997, 101 (16) :2953-2959

[10]

von Sonntag C., 1987, CHEM BASIS RAD BIOL