Sub-lethal damage of Listeria monocytogenes after long-term chilled storage at 4°C

被引:21
作者
Dykes, GA [1 ]
Withers, KM [1 ]
机构
[1] MIRINZ Food Technol & Res Ltd, Hamilton, New Zealand
关键词
D O I
10.1046/j.1365-2672.1999.00472.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The possibility that long term in vitro chilled storage may result in sub-lethal damage to Listeria monocytogenes cells was investigated by comparing growth of chill-stored (starvation at 4 degrees C) and fresh cultures on selective and non-selective media. Growth of freshly grown cells was minimally (3-8%) affected by selective LSAMM agar compared with non-selective Brain Heart Infusion agar. In contrast, numbers of chill-stored strains were reduced by greater than 99% after direct plating on the same selective and non-selective media. Furthermore, chill-stored strains were able to grow in standard selective broth (Listeria Selective broth and Fraser broth) only if undiluted inocula (approximately 10(5)-10(6) cfu ml(-1)) were used, whereas they were capable of growth in Brain Heart Infusion broth even when the lowest dilutions were used (approximately 10(1) cfu ml(-1)). The potential public health consequences of this finding for the isolation of Listeria monocytogenes from foods is considered.
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页码:45 / 48
页数:4
相关论文
共 17 条
[1]  
Avery SM, 1995, INT J FOOD SCI TECH, V30, P725
[2]   Differences in pathogenicity for chick embryos and growth kinetics at 37 degrees C between clinical and meat isolates of Listeria monocytogenes previously stored at 4 degrees C [J].
Avery, SM ;
Buncic, S .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1997, 34 (03) :319-327
[3]   A TECHNIQUE FOR THE DIRECT IDENTIFICATION OF HAMOLYTIC-PATHOGENIC LISTERIA ON SELECTIVE PLATING MEDIA [J].
BLANCO, M ;
FERNANDEZGARAYZABAL, JF ;
DOMINGUEZ, L ;
BRIONES, V ;
VAZQUEZBOLAND, JA ;
BLANCO, JL ;
GARCIA, JA ;
SUAREZ, G .
LETTERS IN APPLIED MICROBIOLOGY, 1989, 9 (04) :125-128
[4]   Listeriolysin O production and pathogenicity of non-growing Listeria monocytogenes stored at refrigeration temperature [J].
Buncic, S ;
Avery, SM ;
Rogers, AR .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1996, 31 (1-3) :133-147
[5]   Relationship between variations in pathogenicity and lag phase at 37 degrees C of Listeria monocytogenes previously stored at 4 degrees C [J].
Buncic, S ;
Avery, SM .
LETTERS IN APPLIED MICROBIOLOGY, 1996, 23 (01) :18-22
[6]   DEVELOPMENT OF A REPAIR-ENRICHMENT BROTH FOR RESUSCITATION OF HEAT-INJURED LISTERIA-MONOCYTOGENES AND LISTERIA-INNOCUA [J].
BUSCH, SV ;
DONNELLY, CW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (01) :14-20
[7]   BACTERIAL STARVATION STRESS AND CONTAMINATION OF BEEF [J].
DICKSON, JS ;
FRANK, JF .
FOOD MICROBIOLOGY, 1993, 10 (03) :215-222
[8]   ENHANCED RECOVERY OF LISTERIA FROM DAIRY-PLANT PROCESSING ENVIRONMENTS THROUGH COMBINED USE OF REPAIR ENRICHMENT AND SELECTIVE ENRICHMENT DETECTION PROCEDURES [J].
FLANDERS, KJ ;
PRITCHARD, TJ ;
DONNELLY, CW .
JOURNAL OF FOOD PROTECTION, 1995, 58 (04) :404-409
[9]   INJURY, RESUSCITATION AND DETECTION OF LISTERIA SPP. FROM FROZEN ENVIRONMENTS [J].
FLANDERS, KJ ;
DONNELLY, CW .
FOOD MICROBIOLOGY, 1994, 11 (06) :473-480
[10]  
Franco CM, 1997, ARCH LEBENSMITTELHYG, V48, P54