Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

被引:224
作者
Bogliotti, Yanina Soledad [1 ]
Wu, Jun [2 ,3 ,4 ,5 ]
Vilarino, Marcela [1 ]
Okamura, Daiji [4 ,6 ]
Soto, Delia Alba [1 ]
Zhong, Cuiqing [5 ]
Sakurai, Masahiro [2 ,3 ,4 ,5 ]
Sampaio, Rafael Vilar [1 ]
Suzuki, Keiichiro [5 ]
Belmonte, Juan Carlos Izpisua [5 ]
Ross, Pablo Juan [1 ]
机构
[1] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA
[2] Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 USA
[3] Univ Texas Southwestern Med Ctr Dallas, Hamon Ctr Regenerat Sci & Med, Dallas, TX 75390 USA
[4] Univ Catolica San Antonio Murcia, Murcia 30107, Spain
[5] Salk Inst Biol Studies, Gene Express Lab, La Jolla, CA 92037 USA
[6] Kindai Univ, Grad Sch Agr, Dept Adv Biosci, Nara 6318505, Japan
基金
美国农业部; 巴西圣保罗研究基金会; 美国国家科学基金会;
关键词
bovine; embryonic stem cell; pluripotency; inner cell mass; NAIVE; LINES; CULTURE; STATES; METHYLATION; GENERATION; FEATURES; GENES;
D O I
10.1073/pnas.1716161115
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3-4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.
引用
收藏
页码:2090 / 2095
页数:6
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