Characterization of altered regulation variants of dinitrogenase reductase-activating glycohydrolase from Rhodospirillum rubrum

被引：9

作者：

Kim, K ^{[1
]}

Zhang, YP ^{[1
]}

Roberts, GP ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

来源：

FEBS LETTERS | 2004年 / 559卷 / 1-3期

关键词：

nitrogen fixation; posttranslational regulation; adenosine diphosphate-ribosylation; dinitrogenase reductase-activating glycohydrolase; Rhodospirillum rubrum;

D O I：

10.1016/S0014-5793(04)00031-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Rhodospirillum rubrum, nitrogenase activity is subject to posttranslational regulation through the adenosine diphosphate (ADP)-ribosylation of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase (DRAT) and dinitrogenase reductase-activating glycohydrolase (DRAG). To study the posttranslational regulation of DRAG, its gene was mutagenized and colonies screened for altered DRAG regulation. Three different mutants were found and the DRAG variants displayed different biochemical properties including an altered affinity for divalent metal ions. Taken together, the results suggest that the site involved in regulation is physically near the metal binding site of DRAG. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：84 / 88

页数：5

共 33 条

[1] EPR spectral evidence for a binuclear Mn(II) center in dinitrogenase reductase-activating glycohydrolase from Rhodospirillum rubrum [J].