Chromic gut suture reduces calcitonin-gene-related peptide and substance P levels in the spinal cord following chronic constriction injury in the rat

被引:19
作者
Xu, JY
Pollock, CH
Kajander, KC
机构
[1] UNIV MINNESOTA, DEPT ORAL SCI, MINNEAPOLIS, MN 55455 USA
[2] UNIV MINNESOTA, SCH MED, DEPT PSYCHOL, MINNEAPOLIS, MN 55455 USA
[3] DEPT CELL BIOL & NEUROANAT, MINNEAPOLIS, MN 55455 USA
[4] PROGRAM NEUROSCI, MINNEAPOLIS, MN 55455 USA
关键词
hyperalgesia; neuralgia; neuropeptide; radioimmunoassay; sciatic nerve; suture;
D O I
10.1016/0304-3959(95)00172-7
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
The chronic constriction injury (CCI) is an animal model of an experimental peripheral neuropathy. In this model, a mononeuropathy is produced by loosely ligating the left sciatic nerve of the rat with chromic gut suture (Bennett and Xie 1988). Maves et al. (1993) have proposed that chemical constituents of chromic gut suture influence the behavioral changes of rats with the CCI. Considering their results, we became interested in evaluating whether the type of suture material used to produce the CCI also affected spinal levels of calcitonin-gene-related peptide immunoreactivity (CGRP-ir) and substance P immunoreactivity (SP-ir), peptides that are associated with small primary afferent neurons. Using methods of radioimmunoassay (RIA), we measured levels of CGRP-ir and SP-ir in the dorsal quadrants of approximately the lumbar 4-5 (L4-L5) spinal segments of rats with a CCI induced using polyglactin (Vicryl(R)), plain gut, or chromic gut suture. We observed bilateral decreases in CGRP-ir and SP-ir 60 days after a CCI induced with chromic gut suture, but no changes in peptide levels after a CCI induced with either polyglactin or plain gut suture. These results suggest two possibilities: (1) chromic gut suture, when used to produce the CCI, has more than just a constrictive effect on the sciatic nerve, and/or (2) different suture materials produce changes in CGRP-ir and SP-ir with a differential time-course. Our experiments are unable to distinguish between these two possibilities.
引用
收藏
页码:503 / 509
页数:7
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