Highly efficient single molecule detection in microstructures

被引：22

作者：

Dörre, K

Stephan, J

Lapczyna, M

Stuke, M

Dunkel, H

Eigen, M

机构：

[1] Max Planck Inst Biophys Chem, Abt Biochem Kinet, D-37077 Gottingen, Germany

[2] Max Planck Inst Biophys Chem, Laserphys Abt, D-37077 Gottingen, Germany

来源：

JOURNAL OF BIOTECHNOLOGY | 2001年 / 86卷 / 03期

关键词：

fluorescence single molecule detection; confocal set-up; microstructure; elliptical volume; multi-element; diffusion and flow;

D O I：

10.1016/S0168-1656(00)00415-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

For DNA single molecule sequencing, the complete detection of all dye-labeled monomers which are cleaved off during the sequencing reaction is an essential requirement. In this work we address the feasibility of single molecule detection in microstructures with a confocal multi element set-up. We present statistical data on single molecule recognition and explain a refined data evaluation technique for single molecule burst analysis. From these data the signal-to-noise ratio in microstructures is evaluated as well as the overall detection efficiency. So far, detection efficiencies of single molecule events of up to 60% have been shown in microstructures. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：225 / 236

页数：12

共 32 条

[1] Single molecule fluorescence spectroscopy at ambient temperature [J].