Production of β-mannanase and β-mannosidase from Aspergillus awamori K4 and their properties

beta -Mannanase and beta -mannosidase from Aspergillus awamori K4 was produced by solid culture with coffee waste and wheat bran. The optimum composition for enzyme production was 40% coffee waste-60% wheat bran. Two enzymes were partially purified. Optimum pH was about 5 for both enzymes, and optimum temperature was around 80 degreesC for beta -mannanase and 60-70 degreesC for beta -mannosidase. These enzymes produced some oligosaccharides from glucomannan and galactomannan by their hydrolyzing and transferring activities. beta -Mannanase hydrolyzed konjak and locust bean gum 39.1% and 15.8%, respectively. Oligosaccharides of various molecular size were released from glucomannan of konjak, but on the addition of cellulase, mannobiose was released selectively. In locust bean gum, tetra-, tri-, and disaccharides (mannobiose) were mainly released by K4 beta -mannanase. Tetra- and trisaccharides were heterooligosaccharides consisting of galactose and mannose residues, K4 beta -mannosidase had a transglycosylation action, transferring mannose residue to alcohols and sugars like fructose.