Evaluation of microsatellites as a potential tool for product tracing of ground beef mixtures

被引：23

作者：

Shackell, GH ^{[1
]}

Mathias, HC ^{[1
]}

Cave, VM ^{[1
]}

Dodds, KG ^{[1
]}

机构：

[1] AgRes Ltd, Invermay Agr Ctr, Mosgiel, New Zealand

来源：

MEAT SCIENCE | 2005年 / 70卷 / 02期

关键词：

DNA; microsatellites; traceability; ground beef;

D O I：

10.1016/j.meatsci.2005.01.020

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Microsatellite genotyping was evaluated as a potential tool for DNA-based tracing of ground beef product. DNA from mixtures containing different numbers of individuals was analysed with a set of cattle microsatellite markers frequently used for parentage testing. As samples contained DNA from several animals, the microsatellite markers showed multiple peaks. The method could distinguish between mixtures containing equal amounts of meat from three different individuals, meat from three individuals mixed in different proportions, ground beef mixtures purchased in different cities, and different batches of ground beef patties. Limitations occurred when batches contained large numbers of individuals (> 10) and different batches used meat from the same individuals. We conclude that DNA microsatellites may be useful for DNA traceability of ground beef mixtures prepared from less than 10 individuals, but where larger numbers of animals contribute to a mixture the method is not consistently accurate. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：337 / 345

页数：9

共 27 条

[1] Meat traceability using DNA markers: application to the beef industry [J].