Multilocus sequence typing reveals that Bacillus cereus strains isolated from clinical infections have distinct phylogenetic origins

被引：38

作者：

Barker, M

Thakker, B

Priest, FG ^{[1
]}

机构：

[1] Heriot Watt Univ, Sch Life Sci, Edinburgh EH14 4AS, Midlothian, Scotland

[2] Glasgow Royal Infirm, Dept Med Microbiol, Glasgow, Lanark, Scotland

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 245卷 / 01期

关键词：

Bacillus anthracis; Bacillus cereus; Bacillus thuringiensis; MLST; clone; phylogeny;

D O I：

10.1016/j.femsle.2005.03.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Eight strains of Bacillus cereus isolated from bacteremia and soft tissue infections were assigned to seven sequence types (STs) by multilocus sequence typing (MLST). Two strains from different locations had identical STs. The concatenated sequences of the seven STs were aligned with 65 concatenated sequences from reference STs and a neighbor-joining tree was constructed. Two strains were distantly related to all reference STs. Three strains were recovered in a clade that included Bacillus anthracis, R cereus and rare Bacillus thuringiensis strains while the other three strains were assigned to two STs that were more closely affiliated to most of the B. thuringiensis STs. We conclude that invasive B. cereus strains do not form a single clone or clonal complex of highly virulent strains. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.

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收藏

页码：179 / 184

页数：6

相关论文

共 28 条

[1] Sporulation and δ-endotoxin synthesis by Bacillus thuringiensis [J].

Aronson, A .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2002, 59 (03) :417-425

[2] Bacillus anthracis, a bug with attitude! [J].

Baillie, L ;

Read, TD .

CURRENT OPINION IN MICROBIOLOGY, 2001, 4 (01) :78-81

[3] Clonal relationships between invasive and carriage Streptococcus pneumoniae and serotype- and clone-specific differences in invasive disease potential [J].

Brueggemann, AB ;

Griffiths, DT ;

Meats, E ;

Peto, T ;

Crook, DW ;

Spratt, BG .

JOURNAL OF INFECTIOUS DISEASES, 2003, 187 (09) :1424-1432

[4] Molecular mechanisms of Bacillus endophthalmitis pathogenesis [J].

Callegan, MC ;

Kane, ST ;

Cochran, DC ;

Gilmore, MS .

DNA AND CELL BIOLOGY, 2002, 21 (5-6) :367-373

[5] Multiple sequence alignment with the Clustal series of programs [J].

Chenna, R ;

Sugawara, H ;

Koike, T ;

Lopez, R ;

Gibson, TJ ;

Higgins, DG ;

Thompson, JD .

NUCLEIC ACIDS RESEARCH, 2003, 31 (13) :3497-3500

[6] Emetic toxin formation of Bacillus cereus is restricted to a single evolutionary lineage of closely related strains [J].

Ehling-Schulz, M ;

Svensson, B ;

Guinebretiere, MH ;

Lindbäck, T ;

Andersson, M ;

Schulz, A ;

Fricker, M ;

Christiansson, A ;

Granum, PE ;

Märtlbauer, E ;

Nguyen-The, C ;

Salkinoja-Salonen, M ;

Scherer, S .

MICROBIOLOGY-SGM, 2005, 151 :183-197

[7] Multilocus sequence typing and antigen gene sequencing in the investigation of a meningococcal disease outbreak [J].

Feavers, IM ;

Gray, SJ ;

Urwin, R ;

Russell, JE ;

Bygraves, JA ;

Kaczmarski, EB ;

Maiden, MCJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) :3883-3887

[8] How clonal is Staphylococcus aureus? [J].

Feil, EJ ;

Cooper, JE ;

Grundmann, H ;

Robinson, DA ;

Enright, MC ;

Berendt, T ;

Peacock, SJ ;

Smith, JM ;

Murphy, M ;

Spratt, BG ;

Moore, CE ;

Day, NPJ .

JOURNAL OF BACTERIOLOGY, 2003, 185 (11) :3307-3316

[9] Fatal Bacillus cereus sepsis following resolving neutropenic enterocolitis during the treatment of acute leukemia [J].

Ginsburg, AS ;

Salazar, LG ;

True, LD ;

Disis, ML .

AMERICAN JOURNAL OF HEMATOLOGY, 2003, 72 (03) :204-208

[10] Bacillus cereus and its food poisoning toxins [J].

Granum, PE ;

Lund, T .

FEMS MICROBIOLOGY LETTERS, 1997, 157 (02) :223-228