Two-dimensional nano-liquid chromatography-mass spectrometry system for applications in proteomics

被引：60

作者：

Nägele, E ^{[1
]}

Vollmer, M ^{[1
]}

Hörth, P ^{[1
]}

机构：

[1] Agilent Technol Deutschland GmbH, D-76337 Waldbronn, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 1009卷 / 1-2期

关键词：

proteomics; Escherichia coli; two-dimensional liquid chromatography; proteins;

D O I：

10.1016/S0021-9673(03)01034-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This work demonstrates the development of a method for the analysis of complex proteome samples by two-dimensional nano-liquid chromatography-mass spectrometry. This approach includes strong cation-exchange, sample enrichment, reversed-phase chromatography and nanospray ion trap mass spectroscopy with data dependent tandem mass spectrometry spectra acquisition, and subsequent database search. The new methodology was first evaluated using standard protein digest samples. Finally, data for the analysis of a total Escherichia coli proteome are provided. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：197 / 205

页数：9

共 30 条

[1] Selective analysis of phosphopeptides within a protein mixture by chemical modification, reversible biotinylation and mass spectrometry [J].

Adamczyk, M ;

Gebler, JC ;

Wu, J .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (16) :1481-1488

[2] Automated LC-LC-MS-MS platform using binary ion-exchange and gradient reversed-phase chromatography for improved proteomic analyses [J].

Davis, MT ;

Beierle, J ;

Bures, ET ;

McGinley, MD ;

Mort, J ;

Robinson, JH ;

Spahr, CS ;

Yu, W ;

Luethy, R ;

Patterson, SD .

JOURNAL OF CHROMATOGRAPHY B, 2001, 752 (02) :281-291

[3] A proteomic view of the Plasmodium falciparum life cycle [J].

Florens, L ;

Washburn, MP ;

Raine, JD ;

Anthony, RM ;

Grainger, M ;

Haynes, JD ;

Moch, JK ;

Muster, N ;

Sacci, JB ;

Tabb, DL ;

Witney, AA ;

Wolters, D ;

Wu, YM ;

Gardner, MJ ;

Holder, AA ;

Sinden, RE ;

Yates, JR ;

Carucci, DJ .

NATURE, 2002, 419 (6906) :520-526

[4]

GIDDINGS JC, 1990, J HIGH RES CHROMATOG, V62, P978

[5]

GIGGS L, 2001, J CHROMATOGR A, V924, P359

[6]

Görg A, 2000, ELECTROPHORESIS, V21, P1037, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO

[7]

2-V

[8] Disease proteomics [J].

Hanash, S .

NATURE, 2003, 422 (6928) :226-232

[9]

Issaq HJ, 1999, ELECTROPHORESIS, V20, P1533, DOI 10.1002/(SICI)1522-2683(19990601)20:7<1533::AID-ELPS1533>3.0.CO

[10]

2-V

← 1 2 3 →