Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes

被引：600

作者：

Ford, MGJ ^{[1
]}

Pearse, BMF ^{[1
]}

Higgins, MK ^{[1
]}

Vallis, Y ^{[1
]}

Owen, DJ ^{[1
]}

Gibson, A ^{[1
]}

Hopkins, CR ^{[1
]}

Evans, PR ^{[1
]}

McMahon, HT ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

SCIENCE | 2001年 / 291卷 / 5506期

关键词：

D O I：

10.1126/science.291.5506.1051

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Adaptor protein 180 (AP180) and its homolog, clathrin assembly Lymphoid myeloid Leukemia protein (CALM), are closely related proteins that play important roles in clathrin-mediated endocytosis. Here, we present the structure of the NH2-terminal domain of CALM bound to phosphatidylinositol-4,5-bisphosphate [Ptdlns(4,5)P-2] via a lysine-rich motif. This motif is found in other proteins predicted to have domains of similar structure (for example, Huntingtin interacting protein 1). The structure is in part similar to the epsin NH2-terminal (ENTH) domain, but epsin Lacks the Ptdlns(4,5) P-2-binding site. Because AP180 could bind to Ptdins(4,5)P-2 and clathrin simultaneously, it may serve to tether clathrin to the membrane. This was shown by using purified components and a budding assay on preformed Lipid monolayers. In the presence of AP180, clathrin lattices formed on the monolayer. When AP2 was also present, coated pits were formed.

引用

页码：1051 / 1055

页数：5

共 36 条

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