On-line sample concentration in micellar electrokinetic chromatography with cationic micelles in a coated capillary

被引：45

作者：

Kim, JB ^{[1
]}

Otsuka, K ^{[1
]}

Terabe, S ^{[1
]}

机构：

[1] Himeji Inst Technol, Fac Sci, Kamigori, Hyogo 6781297, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 912卷 / 02期

关键词：

micellar electrokinetic chromatography; sample handling; sample stacking; sweeping; coated capillaries; background electrolyte composition; surfactants; steroids; carboxylic acids; naphthalenedisulfonic acids;

D O I：

10.1016/S0021-9673(01)00599-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The electroosmotic flow was successfully suppressed even in the presence of cationic surfactants, when a polyacrylamide-coated capillary was employed. Two on-line sample concentration techniques of sample stacking and sweeping were evaluated in micellar electrokinetic chromatography (MEKC) using the polyacrylamide-coated capillary. Cationic surfactants were used as pseudostationary phases in MEKC. At least 60-fold and about 600-fold increases in detection sensitivity were obtained in terms of peak heights by sample stacking and sweeping, respectively. (C) 2001 Published by Elsevier Science B.V.

引用

页码：343 / 352

页数：10

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