Protein tyrosine kinase inhibitor, genistein, enhances apoptosis and cell cycle arrest in K562 cells treated with γ-irradiation

被引：44

作者：

Papazisis, KT ^{[1
]}

Zambouli, D ^{[1
]}

Kimoundri, OT ^{[1
]}

Papadakis, ES ^{[1
]}

Vala, V ^{[1
]}

Geromichalos, GD ^{[1
]}

Voyatzi, S ^{[1
]}

Markala, D ^{[1
]}

Destouni, E ^{[1
]}

Boutis, L ^{[1
]}

Kortsaris, AH ^{[1
]}

机构：

[1] Theagen Canc Hosp, Salonika 54007, Greece

来源：

CANCER LETTERS | 2000年 / 160卷 / 01期

关键词：

genistein; gamma-irradiation; K562; apoptosis; cell cycle;

D O I：

10.1016/S0304-3835(00)00569-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Genistein, is a natural isoflavone compound with a potent activity against protein tyrosine kinases. The leukemic cell line, K562. is a bcr/abl (Philadelphia chromosome) positive cell line that is resistant to DNA-damaging agents, including gamma -irradiation. Treatment with genistein increased apoptosis and promoted G2-phase arrest in the non-apoptotic population of the y-irradiated K562 cells. Irradiated cells that survived 77- h after the irradiation had a normal distribution in cell cycle, whilst genistein treatment kept cells arrested in the G2-phase, decreased the S-phase fraction and suppressed DNA-synthesis. Taken together, our results show that genistein augments apoptotic cell death after gamma -irradiation in K562 cells and this result cannot be attributed to abrogation of the G2/M checkpoint. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：107 / 113

页数：7

共 22 条

[1]

AKIYAMA T, 1987, J BIOL CHEM, V262, P5592

[2] Bcl-2-independent Bcr-Abl-mediated resistance to apoptosis:: protection is correlated with up regulation of Bcl-xL [J].