Antigen-specific and non-specific CD4+ T cell recruitment and proliferation during influenza infection

被引:65
作者
Chapman, TJ
Castrucci, MR
Padnick, RC
Bradley, LM
Topham, DJ
机构
[1] Univ Rochester, Ctr Med, Dept Microbiol & Immunol,Aab Inst Biomed Sci, David H Smith Ctr Vaccine Biol & Immunol, Rochester, NY 14642 USA
[2] Ist Super Sanita, I-00161 Rome, Italy
[3] Sidney Kimmel Canc Ctr, San Diego, CA 92121 USA
关键词
antigen; CD4(+) T cell; influenza;
D O I
10.1016/j.virol.2005.06.023
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To track epitope-specific CD4(+) T cells at a single-cell level during influenza infection, the MHC class II-restricted OVA(323-339) epitope was engineered into the neuraminidase stalk of influenza/AIWSN, creating a surrogate viral antigen. The recombinant virus, influenza A/WSN/OVA(II), replicated well, was cleared normally, and stimulated both wild-type and DO11.10 or OT-II TCR transgenic OVA-specific CD4(+) T cells. OVA-specific CD4 T cells proliferated during infection only when the OVA epitope was present. However, previously primed (but not naive) transgenic CD4(+) T cells were recruited to the infected lung both in the presence and absence of the OVA323-339 epitope. These data show that, when primed, CD4(+) T cells may traffic to the lung in the absence of antigen, but do not proliferate. These results also document a useful tool for the study of CD4 T cells in influenza infection. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:296 / 306
页数:11
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