Heterotypic interaction of CRTAM with Necl2 induces cell adhesion on activated NK cells and CD8+ T cells

被引:60
作者
Arase, N
Takeuchi, A
Unno, M
Hirano, S
Yokosuka, T
Arase, H
Saito, T [1 ]
机构
[1] RIKEN, Res Ctr Allergy & Immunol, Lab Cell Signaling, Yokohama, Kanagawa 2300045, Japan
[2] Chiba Univ, Grad Sch Med, Dept Mol Genet, Chiba 2608670, Japan
[3] Osaka Univ, Dept Immunochem, Microbial Dis Res Inst, Suita, Osaka, Japan
[4] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan
关键词
cell adhesion; expression cloning; Ig superfamily; subtraction;
D O I
10.1093/intimm/dxh299
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
NK cells and CD8(+) T cells exhibit cytotoxicity and cytokine production upon recognizing target cells through cell-cell interaction. We screened the molecules involved in the recognition and regulation of these cells using cDNA subtraction between naive and activated NK cells. We identified class I-restricted T cell-associated molecule (CRTAM), a two Ig domain-bearing surface receptor, as a molecule rapidly and transiently expressed on NK cells and CD8(+) T cells upon activation. CRTAM is expressed as a dimer on the cell surface, and its expression is transcriptionally regulated. Using an expression-cloning system, we then further identified Nectin-like (Necl) molecule 2, a three Ig domain-containing receptor, as a ligand of CRTAM. While Necl2 mediates homotypic interaction, CRTAM interacts with Necl2 but not with CRTAM itself. The heterotypic CRTAM-Necl2 interaction has a higher affinity than the homotypic Necl2 interaction. Although there was no clear alteration in the cytotoxic function of the NK cells and CD8(+) T cells against the Necl2-expressing target cells, T cells expressing CRTAM tightly bound to Necl2-expressing cells. CRTAM(+) cells did not induce homotypic aggregation but they did exert strong heterotypic binding with Necl2(+) cells, which was inhibited by the addition of the CRTAM-Ig fusion protein. These results suggest that the heterotypic interaction between CRTAM and Necl2 plays an important role in the adhesion, interaction or migration of NK cells and CD8(+) T cells upon stimulation.
引用
收藏
页码:1227 / 1237
页数:11
相关论文
共 34 条
[1]   Lipopolysaccharide interaction with cell surface toll-like receptor 4-MD-2: Higher affinity than that with MD-2 or CD14 [J].
Akashi, S ;
Saitoh, S ;
Wakabayashi, Y ;
Kikuchi, T ;
Takamura, N ;
Nagai, Y ;
Kusumoto, Y ;
Fukase, K ;
Kusumoto, S ;
Adachi, Y ;
Kosugi, A ;
Miyake, K .
JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 198 (07) :1035-1042
[2]   Direct recognition of cytomegalovirus by activating and inhibitory NK cell receptors [J].
Arase, H ;
Mocarski, ES ;
Campbell, AE ;
Hill, AB ;
Lanier, LL .
SCIENCE, 2002, 296 (5571) :1323-1326
[3]   Cutting edge:: The mouse NK cell-associated antigen recognized by DX5 moncoclonal antibody is CD49b (α2 integrin, very late antigen-2) [J].
Arase, H ;
Saito, T ;
Phillips, JH ;
Lanier, LL .
JOURNAL OF IMMUNOLOGY, 2001, 167 (03) :1141-1144
[4]   LFA-1 contributes an early signal for NK cell cytotoxicity [J].
Barber, DF ;
Faure, M ;
Long, EO .
JOURNAL OF IMMUNOLOGY, 2004, 173 (06) :3653-3659
[5]   Coexpression of CD58 or CD48 with intercellular adhesion molecule 1 on target cells enhances adhesion of resting NK cells [J].
Barber, DF ;
Long, EO .
JOURNAL OF IMMUNOLOGY, 2003, 170 (01) :294-299
[6]  
BIEDERER T, SCIENCE, V297, P1525
[7]   Identification of PVR (CD155) and nectin-2 (CD112) as cell surface ligands for the human DNAM-1 (CD226) activating molecule [J].
Bottino, C ;
Castriconi, R ;
Pende, D ;
Rivera, P ;
Nanni, M ;
Carnemolla, B ;
Cantoni, C ;
Grassi, J ;
Marcenaro, S ;
Reymond, N ;
Vitale, M ;
Moretta, L ;
Lopez, M ;
Moretta, A .
JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 198 (04) :557-567
[8]   Natural killer cells, viruses and cancer [J].
Cerwenka, A ;
Lanier, LL .
NATURE REVIEWS IMMUNOLOGY, 2001, 1 (01) :41-49
[9]   Natural killer cell activation in mice and men: different triggers for similar weapons? [J].
Colucci, F ;
Di Santo, JP ;
Leibson, PJ .
NATURE IMMUNOLOGY, 2002, 3 (09) :807-813
[10]   The innate immune response to tumors and its role in the induction of T-cell immunity [J].
Diefenbach, A ;
Raulet, DH .
IMMUNOLOGICAL REVIEWS, 2002, 188 :9-21