Pleiotropic effects of puf interposon mutagenesis on carotenoid biosynthesis in Rubrivivax gelatinosus - A new gene organization in purple bacteria

被引：45

作者：

Ouchane, S ^{[1
]}

Picaud, M ^{[1
]}

Vernotte, C ^{[1
]}

ReissHusson, F ^{[1
]}

Astier, C ^{[1
]}

机构：

[1] UNIV PARIS 06, CTR GENET MOL, CNRS, UPR 9061, F-91198 GIF SUR YVETTE, FRANCE

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1997年 / 272卷 / 03期

关键词：

D O I：

10.1074/jbc.272.3.1670

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rubrivivax gelatinosus mutants affected in the carotenoid biosynthesis pathways were created by interposon mutagenesis within the puf operon. Genetic and biochemical analysis of several constructed mutants suggest that at least crtC is localized downstream of the puf operon and that it is cotranscribed with this operon. Sequence analysis confirmed the genetic data and showed the presence of crtD and crtC genes downstream of the puf operon, a localization different from that known for other purple bacteria. Inactivation of the crtD gene indicated that the two crt genes are cotranscribed and that they are involved not only in the hydroxyspheroidene biosynthesis pathway as in Rhodobacter sphaeroides and R. capsulatus, but also in the spirilloxanthin biosynthesis pathway. Carotenoid genes implicated in the spirilloxanthin biosynthesis pathway were thus identified for the first time. Furthermore, analysis of carotenoid synthesis in the mutants gave genetic evidence that crtD and crtC genes are cotranscribed with the puf operon using the oxygen regulated puf promoter.

引用

页码：1670 / 1676

页数：7

共 40 条

[1] REACTION CENTER LIGHT HARVESTING B875 COMPLEXES FROM RHODOCYCLUS-GELATINOSUS - CHARACTERIZATION AND IDENTIFICATION OF QUINONES [J].