New mass spectrometric methods for the quantification of protein-ligand binding in solution

被引：20

作者：

Breuker, K ^{[1
]}

机构：

[1] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 01期

关键词：

analytical chemistry; binding assays; mass spectrometry; protein-ligand binding;

D O I：

10.1002/anie.200301695

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

At first sight, it is not immediately discernible how molecular-weight measurements can be used for the determination of binding constants for protein-ligand complexes. Nevertheless, successful strategies have been developed for the quantitative analysis of protein-ligand binding by mass spectrometry (see scheme), which offers the distinct advantages of speed and the potential for automation.

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页码：22 / 25

页数：4

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