Widespread Expression of γ-Glutamyl Cyclotransferase Suggests It Is Not a General Tumor Marker

被引：19

作者：

Amano, Tomonari ^{[2
]}

Eishi, Yoshinobu ^{[2
]}

Yamada, Tetsuo ^{[2
]}

Uchida, Keisuke ^{[2
]}

Minegishi, Kana ^{[2
]}

Tamura, Tomoki ^{[2
]}

Kobayashi, Daisuke ^{[2
]}

Hiroshi, Kawachi ^{[2
]}

Suzuki, Takashige ^{[2
]}

Board, Philip G. ^{[1
]}

机构：

[1] Australian Natl Univ, John Curtin Sch Med Res, Canberra, ACT 2601, Australia

[2] Tokyo Med & Dent Univ, Dept Human Pathol, Tokyo, Japan

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2012年 / 60卷 / 01期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

gamma-glutamyl cyclotransferase; GGCT; cellular distribution; protein expression; gene expression; cancer marker; HUMAN-ERYTHROCYTES; CANCER BIOMARKER; C7ORF24; GLUTAMYLCYCLOTRANSFERASE; GLUTATHIONE; CYCLE;

D O I：

10.1369/0022155411428468

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

gamma-Glutamyl cyclotransferase (GGCT) contributes to the gamma-glutamyl cycle that regulates glutathione metabolism. Although GGCT has been implicated in several studies as a possible cancer marker, little is known about its distribution in cells and tissues. The authors investigated GGCT expression in normal tissues and tumors using Western blots and immunohistochemistry with a novel anti-GGCT monoclonal antibody. GGCT was detected in most organs and was mainly found in epithelial cells. Although the intracellular distribution was mainly cytoplasmic, in some situations, nuclear staining was strong. A significant increase in the expression of GGCT was found in tumors of the lung, esophagus, stomach, bile duct, and uterine cervix. In contrast, there was a significant decrease in expression in renal and urothelial tumors. These results suggest that GGCT may be a biomarker of tumors in a limited range of organs. (J Histochem Cytochem 60:76-86, 2012)

引用

页码：76 / 86

页数：11

共 18 条

[1]

[Anonymous], 1988, Antibodies: A laboratory manual

[2]

Localization and characterization of γ-glutamyl cyclotransferase in cancer cells [J].

Azumi, Kaoru ;

Ikeda, Youhei ;

Takeuchi, Tomoharu ;

Nomura, Tsuyoshi ;

Sabau, Sorin V. ;

Hamada, Jun-Ichi ;

Okada, Futoshi ;

Hosokawa, Masuo ;

Yokosawa, Hideyoshi .

MOLECULAR MEDICINE REPORTS, 2009, 2 (03) :385-391

[3]

ELECTROPHORETIC INVESTIGATION OF GAMMA-GLUTAMYL-CYCLOTRANSFERASE FROM HUMAN-ERYTHROCYTES [J].

BOARD, PG .

HUMAN HEREDITY, 1980, 30 (04) :248-250

[4]

PURIFICATION AND PROPERTIES OF GAMMA-GLUTAMYLCYCLOTRANSFERASE FROM HUMAN ERYTHROCYTES [J].

BOARD, PG ;

MOORE, KA ;

SMITH, JE .

BIOCHEMICAL JOURNAL, 1978, 173 (02) :427-231

[5]

Proteomic Profiling of Mammary Carcinomas Identifies C7orf24, a γ-Glutamyl Cyclotransferase, as a Potential Cancer Biomarker [J].

Gromov, Pavel ;

Gromova, Irina ;

Friis, Esbern ;

Timmermans-Wielenga, Vera ;

Rank, Fritz ;

Simon, Ronald ;

Sauter, Guido ;

Moreira, Jose M. A. .

JOURNAL OF PROTEOME RESEARCH, 2010, 9 (08) :3941-3953

[6]

A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma [J].

Kageyama, Susumu ;

Iwaki, Hideaki ;

Inoue, Hirokazu ;

Isono, Takahiro ;

Yuasa, Takeshi ;

Nogawa, Masaki ;

Maekawa, Taira ;

Ueda, Masamichi ;

Kajita, Yoichiro ;

Ogawa, Osamu ;

Toguchida, Junya ;

Yoshiki, Tatsuhiro .

PROTEOMICS CLINICAL APPLICATIONS, 2007, 1 (02) :192-199

[7]

Laemmli UK, 1970, NATURE, V15, P680, DOI DOI 10.1038/227680A0

[8]

GLUTATHIONE [J].

MEISTER, A ;

ANDERSON, ME .

ANNUAL REVIEW OF BIOCHEMISTRY, 1983, 52 :711-760

[9]

ENZYMOLOGY OF AMINO-ACID TRANSPORT [J].

MEISTER, A .

SCIENCE, 1973, 180 (4081) :33-39

[10]

The identification and structural characterization of C7orf24 as γ-glutamyl cyclotransferase -: An essential enzyme in the γ-glutamyl cycle [J].

Oakley, Aaron J. ;

Yamada, Tetsuo ;

Liu, Dan ;

Coggan, Marjorie ;

Clark, Alan G. ;

Board, Philip G. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (32) :22031-22042

← 1 2 →