Direct visualization of A-, P-, and E-site transfer RNAs in the Escherichia coli ribosome

被引：187

作者：

Agrawal, RK

Penczek, P

Grassucci, RA

Li, YH

Leith, A

Nierhaus, KH

Frank, J

机构：

[1] NEW YORK STATE DEPT HLTH, WADSWORTH CTR LABS & RES, ALBANY, NY 12201 USA

[2] SUNY ALBANY, DEPT BIOMED SCI, ALBANY, NY 12222 USA

[3] MAX PLANCK INST MOLEC GENET, D-14195 BERLIN, GERMANY

来源：

SCIENCE | 1996年 / 271卷 / 5251期

关键词：

D O I：

10.1126/science.271.5251.1000

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transfer RNA (tRNA) molecules play a crucial role in protein biosynthesis in all organisms. Their interactions with ribosomes mediate the translation of genetic messages into polypeptides. Three tRNAs bound to the Escherichia coli 70S ribosome were visualized directly with cryoelectron microscopy and three-dimensional reconstruction, The detailed arrangement of A- and P-site tRNAs inferred from this study allows localization of the sites for anticodon interaction and peptide bond formation on the ribosome.

引用

页码：1000 / 1002

页数：3

共 35 条

[1] BEYER D, 1994, J BIOL CHEM, V269, P30713
[2] BRIMACOMBE R, 1995, EUR J BIOCHEM, V230, P365
[3] BURMA DP, 1986, STRUCTURE FUNCTION G, P438
[4] A MODEL OF PROTEIN-SYNTHESIS BASED ON CRYOELECTRON MICROSCOPY OF THE E-COLI RIBOSOME
FRANK, J
ZHU, J
PENCZEK, P
LI, YH
SRIVASTAVA, S
VERSCHOOR, A
RADERMACHER, M
GRASSUCCI, R
LATA, RK
AGRAWAL, RK
[J]. NATURE, 1995, 376 (6539) : 441 - 444
[5] GORNICKI P, 1984, J BIOL CHEM, V259, P493
[6] GRAJEVSKAJA RA, 1982, EUR J BIOCHEM, V128, P47
[7] DISTANCE MOVED BY TRANSFER-RNA DURING TRANSLOCATION FROM THE A SITE TO THE P SITE ON THE RIBOSOME
JOHNSON, AE
ADKINS, HJ
MATTHEWS, EA
CANTOR, CR
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1982, 156 (01) : 113 - 140
[8] KILL R, 1984, BIOCHEMISTRY-US, V25, P3245
[9] 3-DIMENSIONAL TERTIARY STRUCTURE OF YEAST PHENYLALANINE TRANSFER-RNA
KIM, SH
SUDDATH, FL
QUIGLEY, GJ
MCPHERSON, A
SUSSMAN, JL
WANG, AHJ
SEEMAN, NC
RICH, A
[J]. SCIENCE, 1974, 185 (4149) : 435 - 440
[10] QUANTITATIVE STUDY OF INTERACTION OF DEACYLATED TRANSFER-RNA WITH ESCHERICHIA-COLI RIBOSOMES - ROLE OF 50-S SUBUNITS IN FORMATION OF THE E-SITE
KIRILLOV, SV
MAKAROV, EM
SEMENKOV, YP
[J]. FEBS LETTERS, 1983, 157 (01) : 91 - 94

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