Identification of novel simple sequence length polymorphisms (SSLPs) in mouse by interspersed repetitive element (IRE)-PCR

被引:8
作者
Detter, JC
Nguyen, QA
Kingsmore, SF
机构
[1] Univ Florida, Dept Med, Gainesville, FL 32610 USA
[2] Univ Florida, Ctr Mammalian Genet, Gainesville, FL 32610 USA
[3] CuraGen Corp, New Haven, CT 06511 USA
关键词
D O I
10.1093/nar/26.17.4091
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Interspersed repetitive element (IRE)-PCR is a useful method for identification of novel human or mouse sequence tagged sites (STSs) from contigs of. genomic clones. We describe the use of IRE-PCR with mouse B1 repetitive element primers to generate novel, PCR amplifiable, simple sequence length polymorphisms (SSLPs)from yeast artificial chromosome (YAC) clones containing regions of mouse chromosomes 13 and 14. Forty-two IRE-PCR products were cloned and sequenced from eight YACs. Of these, 29 clones contained multiple simple sequence repeat units. PCR analysis with primers derived from unique sequences flanking the simple sequence repeat units in seven clones showed all to be polymorphic between various mouse strains. This novel approach to SSLP identification represents an efficient method for saturating a genomic interval with polymorphic genetic markers that may expedite the positional cloning of genes for traits and disease's.
引用
收藏
页码:4091 / 4092
页数:2
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