Chymase processes big-endothelin-2 to endothelin-2-(1-31) that induces contractile responses in the isolated monkey trachea

被引：28

作者：

Takai, S ^{[1
]}

Shiota, N ^{[1
]}

Jin, D ^{[1
]}

Miyazaki, M ^{[1
]}

机构：

[1] Osaka Med Coll, Dept Pharmacol, Takatsuki, Osaka 5698686, Japan

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1998年 / 358卷 / 03期

关键词：

chymase; big-endothelin-1; big-endothelin-2; constriction (monkey);

D O I：

10.1016/S0014-2999(98)00622-0

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Purified monkey chymase cleaved the Tyr(31)-Gly(32) bond of big-endothelin-1 and big-endothelin-2 to yield endothelin-1-(1-31) and endothelin-2-(1-31), respectively. In the isolated monkey trachea, endothelin-1-(1-31) and endothelin-2-(1-31), as well as big-endothelin-1 and big-endothelin-2, induced contractile responses. Chymostatin, which inhibits chymase, suppressed the contractile response induced by big-endothelin-2 to 16.6% but not the responses induced by big-endothelin-l, endothelin-1-(1-31) and endothelin-2-(1-31). These results suggest that the contractile response of big-endothelin-2 is predominantly dependent on the conversion of big-endothelin-2 to endothelin-2-(1-31) by chymase. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：229 / 233

页数：5

共 10 条

[1] ENDOTHELIN-CONVERTING ENZYME-2 IS A MEMBRANE-BOUND, PHOSPHORAMIDON-SENSITIVE METALLOPROTEASE WITH ACIDIC PH OPTIMUM [J].

EMOTO, N ;

YANAGISAWA, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (25) :15262-15268

[2] Hydrolysis of big endothelin-1 by a serine protease in the membrane fraction of human lung [J].

Hanson, GC ;

Andersson, KE ;

Gyllstedt, E ;

Hogestatt, ED ;

Lindberg, BF .

REGULATORY PEPTIDES, 1997, 68 (01) :63-69

[3]

LETRONG H, 1987, P NATL ACAD SCI USA, V84, P364

[4] DISTRIBUTION OF CHYMASE-CONTAINING MAST-CELLS IN HUMAN BRONCHI [J].

MATIN, R ;

TAM, EK ;

NADEL, JA ;

CAUGHEY, GH .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1992, 40 (06) :781-786

[5]

Nakano A, 1997, J IMMUNOL, V159, P1987

[6] MARKED SPECIES-DIFFERENCE IN THE VASCULAR ANGIOTENSIN II-FORMING PATHWAYS - HUMANS VERSUS RODENTS [J].

OKUNISHI, H ;

OKA, Y ;

SHIOTA, N ;

KAWAMOTO, T ;

SONG, K ;

MIYAZAKI, M .

JAPANESE JOURNAL OF PHARMACOLOGY, 1993, 62 (02) :207-210

[7] CHARACTERIZATION OF PHOSPHORAMIDON-SENSITIVE METALLOPROTEINASES WITH ENDOTHELIN-CONVERTING ENZYME-ACTIVITY IN PORCINE LUNG MEMBRANE [J].

SAWAMURA, T ;

SHINMI, O ;

KISHI, N ;

SUGITA, Y ;

YANAGISAWA, M ;

GOTO, K ;

MASAKI, T ;

KIMURA, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1161 (2-3) :295-302

[8] Purification and characterization of angiotensin II-generating chymase from hamster cheek pouch [J].

Takai, S ;

Shiota, N ;

Yamamoto, D ;

Okunishi, H ;

Miyazaki, M .

LIFE SCIENCES, 1996, 58 (07) :591-597

[9] Induction of chymase that forms angiotensin II in the monkey atherosclerotic aorta [J].

Takai, S ;

Shiota, N ;

Kobayashi, S ;

Matsumura, E ;

Miyazaki, M .

FEBS LETTERS, 1997, 412 (01) :86-90

[10]

URATA H, 1990, J BIOL CHEM, V265, P22348

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