Extensive chromosome aberrations in Spiroplasma citri strain BR3

被引:32
作者
Ye, FC
Melcher, U
Rascoe, JE
Fletcher, J
机构
[1] OKLAHOMA STATE UNIV, DEPT PLANT PATHOL, STILLWATER, OK 74078 USA
[2] OKLAHOMA STATE UNIV, DEPT BIOCHEM & MOL BIOL, STILLWATER, OK 74078 USA
关键词
Spiroplasma citri; chromosome aberrations; SpV1-like sequences; transposase and insect transmission;
D O I
10.1007/BF02399947
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genetic variations in the plant pathogen, Spiroplasma citri strain BR3, were characterized through physical genome mapping of the original isolate, BR3-3X, and two derivatives, BR3-T and BR3-G, obtained after several years of different maintenance conditions. BR3-T was transmitted from plant to plant via its natural insect vector, the leafhopper Circulifer tenellus, while BR3-G was maintained only in plants by periodic grafting and has lost its ability to be insect transmitted. By pulsed field gel electrophoresis (PFGE) analysis and DNA hybridization, extensive changes in chromosomal DNA restriction patterns relative to the parent, BR3-3X, were observed in both BR3-T and BR3-G each of which also had a larger genome size than the parent line. Genetic organization was relatively conserved between BR3-T and BR3-3X. lit contrast, a large chromosomal inversion and deletions of approximately 10 kb near each of the inversion borders were observed in BR3-G. One of the deletions, which included several possibly functional genes, was closely linked to a SpV1-related transposase gene. The locations of the deletion borders were also determined. The results of this study demonstrated remarkable genome instability of spiroplasmas.
引用
收藏
页码:269 / 286
页数:18
相关论文
共 21 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]   HETEROGENEITY OF GENOME SIZES WITHIN THE GENUS SPIROPLASMA [J].
CARLE, P ;
LAIGRET, F ;
TULLY, JG ;
BOVE, JM .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1995, 45 (01) :178-181
[3]   ORGANIZATION AND NUCLEOTIDE-SEQUENCES OF THE SPIROPLASMA-CITRI GENES FOR RIBOSOMAL PROTEIN-S2, ELONGATION FACTOR-TS, SPIRALIN, PHOSPHOFRUCTOKINASE, PYRUVATE-KINASE, AND AN UNIDENTIFIED PROTEIN [J].
CHEVALIER, C ;
SAILLARD, C ;
BOVE, JM .
JOURNAL OF BACTERIOLOGY, 1990, 172 (05) :2693-2703
[4]  
CHRISTIANSEN G, 1987, ISRAEL J MED SCI, V23, P595
[5]  
CITTI C, 1992, THESIS U 2 BORDEAUX
[6]  
Davis R. E., 1979, Proceedings of Republic of China - United States Cooperative Science Seminar on Mycoplasma Diseases of Plants., P59
[7]   Molecular characterization of Spiroplasma citri BR3 lines that differ in transmissibility by the leafhopper Circulifer tenellus [J].
Fletcher, J ;
Shaw, ME ;
Baker, GR ;
Dugan, KJ ;
Ye, FC ;
Sha, YH ;
Zuck, PD ;
Myers, GD .
CANADIAN JOURNAL OF MICROBIOLOGY, 1996, 42 (02) :124-131
[8]   BRITTLE ROOT DISEASE OF HORSERADISH - EVIDENCE FOR AN ETIOLOGICAL ROLE OF SPIROPLASMA-CITRI [J].
FLETCHER, J ;
SCHULTZ, GA ;
DAVIS, RE ;
EASTMAN, CE ;
GOODMAN, RM .
PHYTOPATHOLOGY, 1981, 71 (10) :1073-1080
[9]  
LAIGRET F, 1990, ZBL BAKT S, V20, P435