Bacterial protein toxins inhibiting low-molecular-mass GTP-binding proteins

被引：40

作者：

Just, I ^{[1
]}

Hofmann, F ^{[1
]}

Genth, H ^{[1
]}

Gerhard, R ^{[1
]}

机构：

[1] Hannover Med Sch, Inst Toxikol, D-30625 Hannover, Germany

来源：

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY | 2001年 / 291卷 / 04期

关键词：

ADP-ribosylation; Clostridium difficile; Clostridium botulinum; mono-glucosylation; Rho proteins;

D O I：

10.1078/1438-4221-00127

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Rho GTPases, which belong to the Ras superfamily of low-molecular-mass GTP-binding proteins, are the preferred intracellular targets of bacterial protein toxins. The Rho GTPases RhoA/B/C, Rac1/2 and Cdc42 are the master regulators of the actin cytoskeleton. Clostridium difficile toxins A and B, the causative agents of the antibiotic-associated pseudomembranous colitis, are intracellularly acting cytotoxins which mono-glucosylate the Rho GTPases. Clostridium botulinum C3 toxin, which is not related to the clostridial neurotoxins, catalyses ADP-ribosylation of RhoA/B/C but not of other Rho GTPases. Glucosylation as well as ADP-ribosylation result in functional inactivation of Rho causing disassembly of the actin cytoskeleton.

引用

页码：243 / 250

页数：8

共 59 条

[1] ADP-RIBOSYLATION OF A 21-24 KDA EUKARYOTIC PROTEIN(S) BY C-3, A NOVEL BOTULINUM ADP-RIBOSYLTRANSFERASE, IS REGULATED BY GUANINE-NUCLEOTIDE [J].

AKTORIES, K ;

FREVERT, J .

BIOCHEMICAL JOURNAL, 1987, 247 (02) :363-368

[2] A CHIMERIC TOXIN TO STUDY THE ROLE OF THE 21 KDA GTP BINDING PROTEIN-RHO IN THE CONTROL OF ACTIN MICROFILAMENT ASSEMBLY [J].

AULLO, P ;

GIRY, M ;

OLSNES, S ;

POPOFF, MR ;

KOCKS, C ;

BOQUET, P .

EMBO JOURNAL, 1993, 12 (03) :921-931

[3] The N-terminal part of the enzyme component (C2I) of the binary Clostridium botulinum C2 toxin interacts with the binding component C2II and functions as a carrier system for a Rho ADP-ribosylating C3-like fusion toxin [J].

Barth, H ;

Hofmann, F ;

Olenik, C ;

Just, I ;

Aktories, K .

INFECTION AND IMMUNITY, 1998, 66 (04) :1364-1369

[4] Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells [J].

Bayles, KW ;

Wesson, CA ;

Liou, LE ;

Fox, LK ;

Bohach, GA ;

Trumble, WR .

INFECTION AND IMMUNITY, 1998, 66 (01) :336-342

[5] Rho GTPases and their effector proteins [J].

Bishop, AL ;

Hall, A .

BIOCHEMICAL JOURNAL, 2000, 348 (02) :241-255

[6] Toxins from anaerobic bacteria: specificity and molecular mechanisms of action [J].

Boquet, P ;

Munro, P ;

Fiorentini, C ;

Just, I .

CURRENT OPINION IN MICROBIOLOGY, 1998, 1 (01) :66-74

[7]

Calderón GM, 1998, INFECT IMMUN, V66, P2755

[8] Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases [J].

Caron, E ;

Hall, A .

SCIENCE, 1998, 282 (5394) :1717-1721

[9] THE MAMMALIAN G-PROTEIN RHOC IS ADP-RIBOSYLATED BY CLOSTRIDIUM-BOTULINUM EXOENZYME C-3 AND AFFECTS ACTIN MICROFILAMENTS IN VERO CELLS [J].

CHARDIN, P ;

BOQUET, P ;

MADAULE, P ;

POPOFF, MR ;

RUBIN, EJ ;

GILL, DM .

EMBO JOURNAL, 1989, 8 (04) :1087-1092

[10] Toxins A and B from Clostridium difficile differ with respect to enzymatic potencies, cellular substrate specificities, and surface binding to cultured cells [J].

ChavesOlarte, E ;

Weidmann, M ;

vonEichelStreiber, C ;

Thelestam, M .

JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (07) :1734-1741

← 1 2 3 4 5 6 →