Different responses of free and peptide-bound cross-links to vitamin D and calcium supplementation in elderly women with vitamin D insufficiency

被引:33
作者
Kamel, S
Brazier, M
Rogez, JC
Vincent, O
Maamer, M
Desmet, G
Sebert, JL
机构
[1] LAB HORMONOL, F-80054 AMIENS, FRANCE
[2] SERV RHUMATOL, F-80054 AMIENS, FRANCE
[3] LAB INNOTHERA, F-94111 ARCUEIL, FRANCE
关键词
D O I
10.1210/jc.81.10.3717
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Recent findings have shown that bisphosphonates had different effects on the urinary excretion of free and peptide-bound cross-links. Because of this discrepancy, we investigated the effects of another antiresorptive therapy, i.e. vitamin D (vitD) and calcium (Ca) supplementation (800 IU vit D3 and 1 g elemental calcium daily for 6 months) in elderly women (n = 21, age: 83.5 +/- 1.5 yr) with vitD insufficiency and secondary hyperparathyroidism (mean level 25 hydroxy vitamin D = 3.17 +/- 1.2 ng/mL, mean level of intact parathormone = 45.3 +/- 22.7 pg/mL) on the urinary excretion of free and peptide-bound cross-links. A group of free-living, healthy elderly women (n = 25, age: 76.6 +/- 3.1 yr) with a normal vitD status (mean level of 25 OH D = 23.4 +/- 8.9 ng/mL, intact parathormone = 30.2 +/- 11.2 pg/mL) was simultaneously studied. Bone resorption was assessed by total (T), free (F), peptidyl (P) hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) measured with high performance liquid chromatography, by F-LP determined with enzyme linked immunosorbent assay (iF-LP) and by the N- and C-terminal telopeptides of type I collagen (NTX and Cross-laps) before and after (3 and 6 months) therapy. Comparison of the two groups of elderly women at baseline showed that the urinary excretion of pyridinoline cross-links (T, F, and peptide-bound forms) and of telopeptide fragment of type I collagen were all increased in patients with a low vitD status. Highly significant differences were seen principally for T-HP, F-HP, and F-LP (P < 0.001). Correlation studies between each marker showed that the values of pyridinoline cross-links (T and peptide-bound forms) and of the telopeptide fragments of type I collagen correlated well, but the correlation was slightly less pronounced between free pyridinolines and the other markers. After treatment, the response to therapy was greatest for peptide-bound cross-links assessed by high performance liquid chromatography and for telopeptide fragments of type I collagen (percent change at 6 months: -21% for P-HP P < 0.05, -26% for P-LP P < 0.05, -31% for NTXP < 0.01, and -51% for CLaps P < 0.001). In contrast, free pyridinolines excretion (F-HP and F-LP) assessed by high performance liquid chromatography as well as by immunoassay remained unchanged at 3 and 6 months. Because marked and significant changes were seen with peptide-bound crosslinks only and not with free forms, we conclude that vitD and Ca therapy has the same effects as bisphosphonates on the urinary excretion of free and peptide-bound cross-links. So far, no rational mechanism can be given to explain this discrepancy, and further studies are needed before routine application of these bone collagen degradation products as bone resorption markers.
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页码:3717 / 3721
页数:5
相关论文
共 20 条
[1]  
BLUMSOHN A, 1995, CLIN CHEM, V41, P1592
[2]  
BONDE M, 1994, CLIN CHEM, V40, P2022
[3]   MARKERS OF BONE REMODELING IN THE ELDERLY SUBJECT - EFFECTS OF VITAMIN-D INSUFFICIENCY AND ITS CORRECTION [J].
BRAZIER, M ;
KAMEL, S ;
MAAMER, M ;
AGBOMSON, F ;
ELESPER, I ;
GARABEDIAN, M ;
DESMET, G ;
SEBERT, JL .
JOURNAL OF BONE AND MINERAL RESEARCH, 1995, 10 (11) :1753-1761
[4]   VITAMIN-D(3) AND CALCIUM TO PREVENT HIP-FRACTURES IN ELDERLY WOMEN [J].
CHAPUY, MC ;
ARLOT, ME ;
DUBOEUF, F ;
BRUN, J ;
CROUZET, B ;
ARNAUD, S ;
DELMAS, PD ;
MEUNIER, PJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1992, 327 (23) :1637-1642
[5]   QUANTITATION OF HYDROXYPYRIDINIUM CROSSLINKS IN COLLAGEN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
EYRE, DR ;
KOOB, TJ ;
VANNESS, KP .
ANALYTICAL BIOCHEMISTRY, 1984, 137 (02) :380-388
[6]   CROSS-LINKING IN COLLAGEN AND ELASTIN [J].
EYRE, DR ;
PAZ, MA ;
GALLOP, PM .
ANNUAL REVIEW OF BIOCHEMISTRY, 1984, 53 :717-748
[7]   ANALYSIS OF PYRIDINOLINE, A CROSS-LINKING COMPOUND OF COLLAGEN-FIBERS, IN HUMAN-URINE [J].
FUJIMOTO, D ;
SUZUKI, M ;
UCHIYAMA, A ;
MIYAMOTO, S ;
INOUE, T .
JOURNAL OF BIOCHEMISTRY, 1983, 94 (04) :1133-1136
[8]   COMPARISON OF NEW BIOCHEMICAL MARKERS OF BONE TURNOVER IN LATE POSTMENOPAUSAL OSTEOPOROTIC WOMEN IN RESPONSE TO ALENDRONATE TREATMENT [J].
GARNERO, P ;
SHIH, WCJ ;
GINEYTS, E ;
KARPF, DB ;
DELMAS, PD .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1994, 79 (06) :1693-1700
[9]  
GARNERO P, 1995, J BONE MINER RES, V10, P641
[10]  
HANSON DA, 1992, J BONE MINER RES, V7, P1251