Selection-driven evolution of emergent dengue virus

被引:166
作者
Bennett, SN [1 ]
Holmes, EC
Chirivella, M
Rodriguez, DM
Beltran, M
Vorndam, V
Gubler, DJ
McMillan, WO
机构
[1] Univ Puerto Rico, Dept Biol, San Juan, PR 00936 USA
[2] Univ Oxford, Dept Zool, Oxford OX1 3PS, England
[3] Univ Puerto Rico, Dept Microbiol & Med Zool, San Juan, PR 00936 USA
[4] Ctr Dis Control & Prevent, San Juan Branch, San Juan, PR USA
[5] Ctr Dis Control & Prevent, Ft Collins, CO USA
关键词
dengue virus; positive selection; epidemiology; phylogeny; maximum likelihood;
D O I
10.1093/molbev/msg182
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
In the last four decades the incidence of dengue fever has increased 30-fold worldwide, and over half the world's population is now threatened with infection from one or more of four co-circulating viral serotypes (DEN-1 through DEN-4). To determine the role of viral molecular evolution in emergent disease dynamics, we sequenced 40% of the genome of 82 DEN-4 isolates collected from Puerto Rico over the 20 years since the onset of endemic dengue on the island. Isolates were derived from years with varying levels of DEN-4 prevalence. Over our sampling period there were marked evolutionary shifts in DEN-4 viral populations circulating in Puerto Rico; viral lineages were temporally clustered and the most common genotype at a particular sampling time often arose from a previously rare lineage. Expressed changes in structural genes did not appear to drive this lineage turnover, even though these regions include primary determinants of viral antigenic properties. Instead, recent dengue evolution can be attributed in part to positive selection on the nonstructural gene 2A (NS2A), whose functions may include replication efficiency and antigenicity. During the latest and most severe DEN-4 epidemic in Puerto Rico, in 1998, viruses were distinguished by three amino acid changes in NS2A that were fixed far faster than expected by drift alone. Our study therefore demonstrates viral genetic turnover within a focal population and the potential importance of adaptive evolution in viral epidemic expansion.
引用
收藏
页码:1650 / 1658
页数:9
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