Linkage map of Japanese black pine based on AFLP and RAPD markers including markers linked to resistance against the pine needle gall midge

被引:55
作者
Hayashi, E [1 ]
Kondo, T
Terada, K
Kuramoto, N
Goto, Y
Okamura, M
Kawasaki, H
机构
[1] Okamura Forest Tree Breeding Ctr, Highett, Vic 3190, Australia
[2] Forest Tree Breeding Ctr, Tohoku Breeding Off, Takisawa, Iwate 0200173, Japan
关键词
Pinus thunbergii; macrogametophyte; linkage map; AFLP; RAPD;
D O I
10.1007/s001220000470
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 [作物学];
摘要
Macrogametophytes derived from the seeds of a tree resistant to pine needle gall midge (PGM) were analyzed using amplified fragment length polymorphism (AFLP). A total of 244 segregating loci were detected among 71 macrogametophytes. Combining the AFLP results with previously reported segregation data for 127 random amplified polymorphic DNA (RAPD) markers, 157 AFLP and 50 RAPD markers with confirmed map positions were assigned to 20 linkage groups and three pairs covering 2085.5 cM with an average distance of 10.1 cM. The total map distance covers about 77.1-78.4% of the total genome, estimated to be approximately 2665-2719 cM in length. Thus, using AFLP markers, the previous RAPD map of this tree was improved in terms of the average distance between markers, the total map distance, and coverage of the genome. Three RAPD markers linked to a gene associated with resistance to PGM were also located on this map.
引用
收藏
页码:871 / 875
页数:5
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