Weak Long-Range Correlated Motions in a Surface Patch of Ubiquitin Involved in Molecular Recognition

被引:137
作者
Bryn Fenwick, R. [2 ]
Esteban-Martin, Santi [2 ]
Richter, Barbara [3 ]
Lee, Donghan [1 ]
Walter, Korvin F. A. [1 ]
Milovanovic, Dragomir [1 ]
Becker, Stefan [1 ]
Lakomek, Nils A. [1 ]
Griesinger, Christian [1 ]
Salvatella, Xavier [2 ,4 ]
机构
[1] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany
[2] Inst Res Biomed IRB Barcelona, Joint BSC IRB Res Programme Computat Biol, Barcelona 08028, Spain
[3] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
[4] ICREA, Barcelona, Spain
基金
英国惠康基金; 欧洲研究理事会;
关键词
RESIDUAL DIPOLAR; BACKBONE MOTION; HYDROGEN-BONDS; DYNAMICS; PROTEIN; ENSEMBLES;
D O I
10.1021/ja200461n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Long-range correlated motions in proteins are candidate mechanisms for processes that require information transfer across protein structures, such as allostery and signal transduction. However, the observation of backbone correlations between distant residues has remained elusive, and only local correlations have been revealed using residual dipolar couplings measured by NMR spectroscopy. In this work, we experimentally identified and characterized collective motions spanning four beta-strands separated by up to 15 angstrom in ubiquitin. The observed correlations link molecular recognition sites and result from concerted conformational changes that are in part mediated by the hydrogen-bonding network.
引用
收藏
页码:10336 / 10339
页数:4
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