A solid-state 14N magic-angle spinning NMR study of some amino acids

Experimental strategies for the acquisition of high-quality N-14 magic-angle spinning (MAS) NMR spectra of the simple amino acids, which exhibit N-14 quadrupole coupling constants (C-Q) on the order of 1.2 MHz, are devised. These are the first useful N-14 MAS spectra reported for nitrogen compounds having a C-Q(N-14) value in excess of 1 MHz. The complete manifolds of spinning sidebands (ssbs), i.e., about 300 ssbs for a spinning frequency of 6.0 kHz, have been observed in the N-14 MAS NMR spectra of a series of amino acids. In their crystal structure these amino acids all exhibit the zwitterionic form and thus the N-14 MAS NMR spectra represent those of a rotating -NH3+ group and not of an amino (-NH2) group. Computer simulations combined with fitting 3 of simulated to the experimental ssb intensities result in the determination of precise values for the N-14 quadrupole coupling (C-Q) and its associated asymmetry parameter (eta(Q)) for the nitrogen sites in these molecules. For some of the amino acids the N-14 MAS NMR spectra exhibit overlap between the manifolds of ssbs from two different nitrogen sites in accordance with their crystal structures. Computer analysis of these spectra results in two different sets of (C-Q, eta(Q)) values which mainly differ in the magnitudes for eta(Q). (C)2003 Elsevier Inc. All rights reserved.