Applications of modern laser technology in art conservation are reviewed with emphasis in the conservation of painted artworks. In particular, processes and critical parameters for the cleaning of paintings by excimer lasers are discussed. The influence of photoxidation or free radical formation processes leading to potential damage is examined in experiments involving model and realistic systems. Laser based spectroscopic techniques for non-destructive pigment and media analysis are also presented. A particular issue in this respect is the applicability of such techniques for on-line control of the cleaning process safeguarding from any damage.